Dasymaschalon leilamericanum (Annonaceae), a new species with evidence of non-monophyly from Mount Lantoy Key Biodiversity Area, Philippines
DOI:
https://doi.org/10.11609/jott.8364.17.3.26571-26586Keywords:
Conservation, Cebu Island, evolutionary, forest, limestone, multivariate, non-monophy, plant, paraphyletic, phylogenetic, sedimentary, shrubAbstract
A new Annonaceae species, Dasymaschalon leilamericanum is described from Mount Lantoy Key Biodiversity Area (KBA) in the Municipality of Argao, Cebu, Philippines. D. leilamericanum is distinguished from closely related species by morphological characters including laminar size, leaf areolation, and seed/fruit shape and size. Significant differences observed in a multivariate analysis of morphological data for D. leilamericanum, D. clusiflorum, D. filipes, D. ellipticum, and D. blumei indicate D. leilamericanum is a distinct species. A phylogenetic tree analysis performed using maturase K (matK) and ribulose 1,5-biphosphate carboxylase (rbcL) as molecular markers failed to match D. leilamericanum with other Dasymaschalon species. Taken together, the results of morphological and molecular analysis indicate a unique evolutionary pathway for D. leilamericanum with its genus.
References
Alström, P., S. Höhna, M. Gelang, P.G.P. Ericson & U. Olsson (2011). Non-monophyly and intricate morphological evolution within the avian family Cettiidae revealed by multilocus analysis of a taxonomically densely sampled dataset.BMC Evolutionar Biology 11:352. https://doi.org/10.1186/1471-2148-11-352
Ast, S. (1938). Anonacées, pp. 59–123. In: Humbert, H. (wd.) Flore Générale de l’Indo-Chine. Suppl. 1. Masson, Paris. https://doi.org/10.5962/bhl.title.44886
Audley-Charles, M.G., D.J. Carter, A.J. Barber, M.S. Norvick & S. Tjokrosapoetro (1979). Reinterpretation of the geology of Seram: implications for the Banda Arc and Northern Australia. Journal of the Geological Society 136: 547–568.
Bân, N.T. (2000). Flora of Vietnam, Vol. 1. Science & Technics Published House, Hanoi, pp. 173–187.
Bân, N.T. (1975). Notes in the genera Dasymaschalon (Hook.f et Thoms) Dalla Torre et Harms (Annonaceae). Botanicheskii Zhurnal 60.2: 224–233.
Cadiz, G.O. & I.E. Buot Jr (2009). An enumeration of the woody plants of Cantipla forest fragments, Cebu Island, Philippines. The Thailand Natural History Museum Journal 4(2): 71–72.
CI/DENR/PAWB-Haribon (2006). Priority Sites for Conservation in the Philippines: Key Biodiversity Areas. Quezon City, Philippines: Conservation International Philippines. 24 pp. Conservation International (CI), Department of Environment and Natural Resources – Protected Areas and Wildlife Bureau (DENR-PAWB), Haribon Foundation for the Conservation of Nature (HARIBON). Retrieved from http://www.conservation.org/global/philippines/publications/Pages/Priority-Sitesfor-Conservation-Key-Biodiversity-Areas.aspx.
Couvreur, T.L.P., M.D. Pirie, L.W. Chatrou, R.M.K. Saunders, Y.C.F. Su, J.E. Richardson & R.H.J. Erkens (2011). Early evolutionary history of the flowering plant family Annonaceae: steady diversification and boreotropical geodispersal. Journal of Biogeography 38: 664–680.
Craib, W.G. (1912). Contributions to the flora of Siam. Bulletin of Miscellaneous Information 3: 144–155. https://doi.org/10.5962/bhl.title.21865
de Vere, N. D., T.C. Rich, S.A. Trinder & C. Long (2015). DNA barcoding for plants. In Plant Genotyping pp. 101–118. Humana Press, New York, 118 pp.
Dalla-Torre, C.W. & H. Harms (1901). Genera siphonogamarum ad systema Englerianum conscripta, Part 3. Engelmann, Leipzig, pp. 172–175. https://doi.org/10.5962/bhl.title.26684
Doyle, J. & A. Le Thomas (2012). Evolution and phylogenetic significance of pollen in Annonaceae. Botanical Journal of the Linnean Society 169: 190–221. https://doi.org/10.1111/j.1095-8339.2012.01241.x
Edgar, R.C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32(5): 1792–1797.
Finet, A. & F. Gagnepain (1906). Contributions à l’étude de la flore de l’Asie orientale. Bulletin de la Société Botanique de France 53 (Mémoires 4): 55–170 + pl. 9–20.
Fries, R.E. (1959). Annonaceae. In: Melchior, H. (Ed.). Die Natürlichen Pflanzenfamilien. Duncker & Humblot, Berlin, pp. 1–170.
Guo, X., D.C. Thomas & R.M.K. Saunders (2018). Gene tree discordance and coalescent methods support ancient intergeneric hybridisation between Dasymaschalon and Friesodielsia (Annonaceae). Molecular Phylogenetics and Evolution 127: 14–29.
Hernandez, J.O., L.S.J. Maldia, D.E. Pulan, I.E. Buot Jr. & B.B. Park (2020). Leaf architecture and petiole anatomy of Philippine Dipterocarpus species (Dipterocarpaceae). Bangladesh Journal of Plant Taxonomy 27(1): 1‒14.
Hooker, J.D. & T. Thomson (1855). Flora Indica: being a systematic account of the plants of British India 1. Pamplin, London, 285 pp. https://doi.org/10.5962/bhl.title.57706
Hutchinson, J. (1923). Contributions towards a phylogenetic system of flowering plants, 2. The genera of Annonaceae. Bulletin of Miscellaneous Information 1923: 241–261. https://doi.org/10.2307/4120580
Kebler, P.J.A. (1993). Annonaceae, pp. 93–129. In: Kubitzki, K., G. Rohwer & V. Bittrich (ed.). The Families and Genera of Vascular Plants: Flowering Plants, Dicotyledons. Magnoliid, Hamanelid and Caryophyllid Families 2. Springer-Verlag, Berlin, 653 pp.
Klucking, E.P. (1986). Leaf venation patterns 1: Annonaceae. J. Cramer, Berlin & Stuttgart.
Koek-Noorman, J., A.K.V. Setten & C.M.V. Zuilen (1997). Studies in Annonaceae XXVI. Flower and fruit morphology in Annonaceae. Their contribution to patterns in cluster analysis. Botanisches Jahrbücher für Systematik, Pflanzengeschichte und Pflanzengeographi 119: 213–230.
Kimura, M. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16:111–120.
Kumar, S., G. Stecher & K. Tamura (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33:1870–1874.
LAWG (1999). Manual of Leaf architecture — Morphological Description and Categorization of Dicotyledonous and Net-Veined Monocotyledonous Angiosperms. Leaf Architecture Working Group, Smithsonian Institution, 65 pp.
Le Thomas, A. (1980). Ultrastructural characters of the pollen grains of African Annonaceae and their significance for the phylogeny of primitive angiosperms: first part. Pollen & Spores 22: 267–342.
Li, P.T. (1993). Novelties in Annonaceae from Asia. Guihaia 13: 311–315.
Lillo, E.P., A.B. Malaki, S.M.T. Alcazar, R.U. Nuevo & R. Rosales (2019). Native Trees on Mount Lantoy Key BiodiversityAreas (KBA), Argao, Cebu, Philippines. Philippine Journal of Science 148(2): 359–371.
Lillo, E.P., A.B. Malaki, S.M.T. Alcazar, B. Redoblado, J.L. Diaz, J.P. Pinote, R. Rosales & I.E. Buot Jr (2020). Native tress in Nug-as forest Key Biodiversity Area, Cebu, Philippines. Biodiversitas 21(9): 4162–4167.
Lillo, E.P., A.B. Malaki, S.M.T. Alcazar, R. Rosales, B.R. Redoblado, J.L.B. Diaz, E.M. Pantinople & I.E. Buot Jr (2021). Inventory of native and mother trees in Key Biodiversity Areas of Cebu Island, Philippines for species selection in local reforestation programs. Biodiversitas 22(11): 4740–4749. https://doi.org/10.13057/biodiv/d221105
Loureiro, J. (1790). Flora Cochinchinensis, Vol. 1. Ulyssipone, Lisbon, 353 pp. https://doi.org/10.5962/bhl.title.40199
Mallari, N.A.D., B.R. Tabaranza Jr. & M.J. Crosby (2001). Key Conservation Sites in the Philippines: A Haribon Foundation and Bird Life International Directory of Important Bird Areas. Lepiten-Tabao M, Gee GA eds. Makati City, Philippines: Bookmark, Inc. 484p.
Malaki, A.B., R.V., Cruz, Bantayan, D.A. Racelis, I.E. Buot, Jr. & L.M. Florece (2018). Factors Affecting the spatial distribution of Black Shama Copsychus cebuensis Steere, 1890 in Argao Watershed Reserve. Philippine Journal of Science 147: 175–189.
Masungsong, L.A., M. Belarmino & I.E. Buot Jr. (2019). Delineation of the selected Cucumis L. species and accessions using leaf architecture characters. Biodiversitas 20: 629–635.
Maxwell, J.F. (1989). Botanical notes on the vascular flora of Chiang Mai Province, Thailand. Natural History Bulletin of the Siam Society 37: 177–185.
Meeran, M., A., Sami, M. Haider, & M. Umar (2023). Multivariate analysis for morphological traits of Amaranthus viridis. Bulletin of Biological and Allied Sciences Research 8: 46. https://doi.org/10.54112/bbasr.v2023i1.46
Merrill, E.D. (1915). Studies on Philippine Annonaceae, I. Philippine Journal of Science, Section C. Botany 10: 227–264.
Merrill, E.D. (1923). An Enumeration of Philippine Flowering Plants, Vol. 2. Manila, Philippines: Bureau of Printing, 530 pp.
Morán-Ordoñez, A. (2020). Conservation of “new” species within and beyond protected areas. Animal Conservation 23: 353–354.
Nei, M. & S. Kumar (2000). Molecular Evolution and Phylogenetics. Oxford University Press, New York. Provide page numbers.
Ngoc-Dai, D.O., L.Ý. Nngoc-Sam, L.Ê. Thi-Huong, B.Á. Troung & Vuongh (2018). Dasymaschalon bachmaensis (Annonaceae), a new species from Bạch Mã National Park, North Central Coast region, Vietnam. Phytotaxa 379(1): 049–056. https://doi.org/10.11646/phytotaxa.379.1.4
Nurmawati, S. (2003). Malesian species of Dasymaschalon (Annonaceae). Floribunda 2(3): 67–81.
Pelser, P.B. & J.F. Barcelona (2017). Base of leaflets. Retrieved from www.phytoimages.siu.edu. Accessed 2 June 2019).
Pi, E.X., Q.F. Peng, H.F. Lu, J.B. Shen. & Y.Q. Du (2009). Leaf morphology and anatomy of Camellia section Camellia (Theaceae). Botanical Journal of the Linnean Society 159: 456–476.
Quimio, J. (2006). Abundance status of flora in Managa-Kotkot-Lusaran watersheds, Cebu, Philippines. Annals of Tropical Research 28(2): 53–75.
Rosales, R.C., E. Lillo, S.M. Alcazar, L. Colita, J. Caballero & A.B. Malaki (2020). Species composition, relative abundance, and distribution of land snail species in Mt. Lantoy Key Biodiversity Area, Cebu, Philippines. Biodiversitas 21: 5438–5447.
Safford, W.E. (1912). Desmos the proper generic name for the so-called Unonas of the Old World. Torrey Botanical Society 39: 501–508. https://doi.org/10.2307/2479122
Simkins, A.T., G.M. Buchanan, R.G. Davies & P.F. Donald (2020). The implications for conservation of a major taxonomic revision of the world’s birds. Animal Conservation 23: 345–352.
Sinclair, J. (1955). A revision of the Malayan Annonaceae. Gardens’ Bulletin Singapore 14: 149–516.
Sun, T.X., H. Wu, P.T. Li, J. Sun & X.F. Zheng (2002). Leaf anatomy of Desmos and Dasymaschalon (Annonaceae) from China in relation to taxonomic significance. Acta Phytotaxonomica Sinica 40: 385–395.
Tran, T.K.P., T.T.T. Vu & S. Widiarsih (2021). Comparison of matK and rbcL DNA barcodes for genetic classification of jewel orchid accessions in Vietnam. Journal of Genetic Engineering and Biotechnology 19: 1–8.
Tsiang, Y. & P.T.Li (1979). Annonaceae, pp. 10–175. In: Tsiang, Y. & P.T. Li (eds.). Flora Reipublicae Popularis Sinicae. Vol. 30 (2). Science Press, Beijing.
Van Heusden, E.C.H. (1992). Flowers of Annonaceae: morphology, classification, and evolution. Blumea Supplement 7: 1–218.
Walker, J.W. (1971). Pollen morphology, phytogeography, and phylogeny of the Annonaceae. Contributions from the Gray Herbarium of Harvard University 202: 1–131.
Wang, J., P. Chalermglin & R.M.K. Saunders (2009). The genus Dasymaschalon (Annonaceae) in Thailand. Systematic Botany 34: 252–265.
Wang, J., D.C. Thomas, Y.C.F. Su, S. Meinke, L.W. Chatrou & R.M.K. Saunders (2012). A plastid DNA phylogeny of Dasymaschalon (Annonaceae) and allied genera: evidence for generic non-monophyly and the parallel evolutionary loss of inner petals. Taxon 61(3): 545–558. https://doi.org/10.1002/tax.613005
Yu, J., J.H. Xue & S.L. Zhou (2011). New universal matK primers for DNA barcoding angiosperms. Journal of Systematics and Evolution 49: 176–181.
Zander, R.H. (2008). Evolutionary inferences from non-monophyly on molecular trees. Taxon 57(4): 1182–1188. https://doi.org/10.1002/tax.574011
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