Morphological variations in marine pufferfish and porcupinefish (Teleostei: Tetraodontiformes) from Tamil Nadu, southeastern coast of India

Main Article Content

K. Kaleshkumar
https://orcid.org/0000-0002-6219-0669
R. Rajaram
https://orcid.org/0000-0003-2621-1677
P. Purushothaman
https://orcid.org/0000-0001-6318-8598
G. Arun
https://orcid.org/0000-0002-1520-0908

Abstract

In the present study, morphological variations in 14 species of two families, Tetraodontidae and Diodontidae, were examined for individuals collected from five different centres in Tamil Nadu in the southeastern coast of India.  Twenty-seven morphological measurements and four meristic characters were taken and used for multivariate analyses such as discriminant function analysis (DFA) & MANOVA.  DFA revealed that the first two functions accounted for more than 75% variation between the species.  Negative allometric values were observed on head length (HL), orbital length (OL), pupil diameter (PD), interorbital length (IOL), pectoral-fin length (PEL), caudal peduncle depth (CPD), dorsal to pectoral fin distance (DPFD), caudal peduncle length (CPL) and post-pectoral-fin length (POPFL) measurements.  Also, MANOVA supported the DFA results.  Additions, allometric relationships, and meristic variations were observed for most of these species.   Moreover, this is the first attempt to describe a greater number of morphological features of the species belonging to the order Tetraodontiformes.

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Author Biographies

K. Kaleshkumar, DNA Barcoding & Marine Genomics Laboratory, Department of Marine Science, School of Marine Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu 620024, India

Mr. K. Kaleshkumar is a PhD research scholar in the Department of Marine Science, whose interest include diversity, distribution, traditional and molecular taxonomy and nutritional evaluation of marine pufferfishes. Currently working on distribution, molecular taxonomy and biomedical applications of marine pufferfishes from Tamil Nadu, south-eastern coast of India. He has seven years of experience in marine pufferfishes. 

R. Rajaram, DNA Barcoding & Marine Genomics Laboratory, Department of Marine Science, School of Marine Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu 620024, India

Dr. R. Rajaram is an Assistant Professor in the Department of Marine Science of Bharathidasan University and his research interest include the ichthyotaxonomy, marine natural products and pollution of marine organisms especially fishes. 

P. Purushothaman, Crustacean Fisheries Division, Central Marine Fisheries Research Institute (CMFRI), Ernakulam North P.O., P.B. No. 1603, Cochin, Kerala 682018, India

Mr. P. Purushothaman is a PhD research scholar in crustacean fisheries division, whose interest includes marine diversity and evolutionary relationships using novel molecular tools. 

G. Arun, DNA Barcoding & Marine Genomics Laboratory, Department of Marine Science, School of Marine Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu 620024, India

Mr. G. Arun is currently a PhD research scholar, whose interest include taxonomy and ecology of marine hydrozoans. He is experienced in Island ecosystem health assessment, coral transplantation, SCUBA diving, and coastal survey.

References

Aktas, M., T. Cemal & A. Bozkurt (2006). Taxonomic description of three shrimp species (Melicertus kerathurus, Metapenaeus monoceros, Penaeus semisulcatus) using multivariate morphometric analyses. Journal of Animal and Veterinary Advances 5(3): 172–175.

Allen, G.R. & J.E. Randall (1977). Review of the Sharpnose Pufferfish (Subfamily: Canthigasterinae) of the Indo-Pacific. Records of the Australian Museum 30: 475–517.

Allen, G. R. & M. V. Erdmann (2012). Reef Fishes of the East Indies: Tropical Reef Research. Perth, Australia. Vol. I-III, 1097–1099pp.

Anonymous (1798). Review of Tome I of ‘Histoire naturelle des poissons’ by Lacepède (1798). Allgemeine Literatur-Zeitung 3 (287): Spalten (columns) 673-680.

Arakawa, O., D.F. Hwang, S. Taniyama & T. Takatani (2010). Toxins of pufferfish that cause human intoxications. Coastal Environmental and Ecosystem Issues of the East China Sea: 227–244. http://hdl.handle.net/10069/23514.

Bloch, M. E. (1785). Auslandische Fische. Berlin, 95, 1801.

Bloch, M.E. & J.G. Schneider. (1801). M.E. Blochii, Systema Ichthyologiae iconibus ex illustratum. Post obitum auctoris opus inchoatum absolvit, correxit, interpolavit Jo. Gottlob Schneider, Saxo. Berolini. Sumtibus Austoris Impressum et Bibliopolio Sanderiano Commissum. i-lx, 584 pp. Berlin.

Bolles, K.L. & G.A. Begg (2000). Distinction between Silver Hake (Merluccius bilinearis) stocks in US waters of the northwest Atlantic based on whole otolith morphometrics. Fishery Bulletin 98(3): 451–462.

Bragadeeswaran, S. & D. Therasa (2010). Biomedical and pharmacological potential of tetrodotoxin-producing bacteria isolated from the marine pufferfish Arothron hispidus (Muller, 1841). The Journal of Venomous Animals and Toxins including Tropical Diseases 16(3): 421–431;

Cadrin, S.X. (2000). Advances in morphometric identification of fishery stocks. Reviews in Fish Biology and Fisheries 10(1): 91–112; https://doi.org/10.1023%2FA%3A1008939104413.

Dekkers, W. J. (1975). Review of the Asiatic freshwater puffers of the genus Tetraodon Linnaeus, 1758 (Pisces, Tetraodontiformes, Tetraodontidae). Bijdragen tot de Dierkunde 45(1): 87-142.

Ferrito, V., M.C. Mannino, A.M. Pappalardo & C. Tigano (2007). Morphological variation among populations of Aphanius fasciatus Nardo, 1827 (Teleostei, Cyprinodontidae) from the Mediterranean. Journal of Fish Biology 70(1): 1–20; https://doi.org/10.1111/j.1095-8649.2006.01192.x

Fiedler, K. (1991). On the task, the measures and the mood in research on affect and social cognition. Emotion and social judgments, 83-104.

Fischer, W. & G. Bianchi, (1984). FAO species identification sheets for fishery purposes: Western Indian Ocean (Fishing Area 51). v. 1: Introductory material. Bony fishes, families: Acanthuridae to Clupeidae.-v. 2: Bony fishes, families: Congiopodidae to Lophotidae.-v. 3: families: Lutjanidae to Scaridae.-v. 4: families: Scatophagidae to Trichiuridae.-v. 5: Bony fishes, families: Triglidae to Zeidae. Chimaeras. Sharks. Lobsters. Shrimps and prawns. Sea turtles. v. 6: Alphabetical index of scientific names and vernacular names.

Fraser-Brunner, A. (1943). Note on the plectognath fishes — VIII. The classification of the suborder Tetraodontoidea, with a synopsis of the genera. Annals & Magazine of Natural History 11(10): 1–18.

Gmelin J.F. (1789). Pisces. In: Caroli a Linné, Systema Naturae per regna tria naturae, secundum classes, ordines, genera, species; cum characteribus, differentiis, synonymis, locis. Editio decimo tertia, aucta, reformata (Gmelin J.F., ed.), pp. 1126-1516. Lipsiae: Georg. Emanuel Beer.

Hamilton, B. (1822). An Account of Fishes found in the River Ganges and its Branches. Archibad Constable & Company, Edinburgh, 405pp.

Hardy, G.S. (1982a). Two new generic names for some Australian pufferfishes (Tetraodontiformes: Tetraodontidae), with species’ redescriptions and osteological comparisons. Australian Journal of Zoology 21: 1–26.

Hardy, G.S. (1982b). First Pacific records of Pelagocephalus marki Heemstra & Smith (Tetraodontiformes: Tetraodontidae), and first male specimen of the genus. New Zealand Journal of Zoology 9: 377–380.

Hardy, G.S. (1983a). Revision of Australian species of Torquigener Whitley (Tetraodontiformes: Tetraodontidae), and two new generic names for Australian puffer fishes. Journal of the Royal Society of New Zealand 13: 1–48.

Hardy, G.S. (1983b). The status of Torquigener hypselogeneion (Bleeker) (Tetraodontiformes: Tetraodontidae) and some related species, including a new species from Hawaii. Pacific Science 37: 65–74.

Hardy, G.S. (1984a). Tylerius, a new generic name for the Indo-Pacific pufferfish, Spheroides spinosissimus Regan, 1908 (Tetraodontiformes: Tetraodontidae) and comparisons with Amblyrhynchotes (Bibron) Dume´ril. Bulletin of Marine Science 35: 32–37.

Hardy, G.S. (1984b). Redescription of the pufferfish Torquigener brevipinnis (Regan) (Tetraodontiformes: Tetraodontidae), with description of a new species of Torquigener from Indonesia. Pacific Science 38: 127–133.

Hirsch, P.E., R. Eckmann, C. Oppelt & J. Behrmannâ€Godel (2013). Phenotypic and genetic divergence within a single whitefish form-detecting the potential for future divergence. Evolutionary applications 6(8): 1119–1132; https://doi.org/10.1111/eva.12087

Hubbs, C.L. & K.F. Lagler (1958). Fishes of the Great Lakes Region, University of Michigan Press, Ann Arbor. 213 pp.

Immaculate, J.S.K., K. Saritha, G. Hermina & P. Jamila (2015). Quality assessment of fresh and dried pufferfish (Lagocephalus lunaris) obtained from Tuticorin, south east coast of India. World Journal of Fish and Marine Sciences 7(4): 268–277.

Jeffares, D.C., C. Rallis, A. Rieux, D. Speed, M. Převorovský, T. Mourier & R. Pracana (2015). The genomic and phenotypic diversity of Schizosaccharomyces pombe. Nature genetics 47(3): 235–241; https://doi.org/10.1038/ng.3215

Kerschbaumer, M., P. Mitteroecker & C. Sturmbauer (2014). Evolution of body shape in sympatric versus non-sympatric Tropheus populations of Lake Tanganyika. Heredity 112(2): 89–98; https:// doi.org/10.1038/hdy.2013.78

Leis, J.M. (1978). Systematics and zeegeography of the porcupinefishes (Diodon, Diodontidae, Tetraodontiformes), with comments on egg and larval development. Fishery Bulletin 76: 535–567.

Leis, J.M. (1984). Tetraodontiformes: relationships. In: Moser HG, Richards WJ, Cohen DM, Fahay MP, Kendall Jr AW, Richardson SL (eds) Ontogeny and systematics of fishes. Am Soc Ichthyol Herpetol Spec Publ 1, pp 459-463.

Linnaeus, C. (1758). Systema Naturae, 10th ed. Systema naturae per regna tria naturae, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis. Tomus I. Editio decima, reformata. Holmiae.

Loy, A., P. Genov, M. Galfo, M.G. Jacobone & A.V. Taglianti (2007). Cranial morphometrics of the Apennine brown bear (Urdus arctos marsicanus) and preliminary notes on the relationships with other southern European populations. Italian Journal of Zoology 75 (1): 67–75; https://doi.org/10.1080/11250000701689857

Matsuura, K. (2000). Tetraodontiformes. In: Randall JE, Lim KKP (eds) A checklist of the fishes of the South China Sea. Raffles Bulletin of Zoology 8: 647-649.

Matsuura, K. (1984). Tetraodontiformes. In: Masuda H, Amaoka K, Araga C, Uyeno T, Yoshino T (1984) The fishes of the Japanese Archipelago. Tokai University Press, Tokyo, pp 356-366, pls 321-334, 370.

Matsuura, K. (1994). Arothron caeruleopunctatus, a new puffer from the Indo-West Pacific. Japanese journal of Ichthyology 41: 29–33.

Matsuura, K. (1999). Taxonomic review of the puffers of the genus Arothron (Tetraodontiformes: Tetraodontidae) with a key to genera of the Indo-West Pacific puffers. Proceedings of 9th Joint Sem Mar Fish Sci: 125–140.

Matsuura, K. (2002). A review of two morphologically similar puffers, Chelonodon laticeps and C. patoca. National Museum of Nature and Science Monographs 22:173-178.

Matsuura, K. (2010). Lagocephalus wheeleri Abe, Tabeta & Kitahama, 1984, a junior synonym of Tetrodon spadiceus Richardson, 1845 (Actinopterygii, Tetraodontiformes, Tetraodontidae). Bulletin of the National Museum of Nature and Science, Series B: 46: 39–46.

Matsuura, K. (2014). A new pufferfish of the genus Torquigener that builds ‘‘mystery circles’’ on sandy bottoms in the Ryukyu Islands, Japan (Actinopterygii: Tetraodontiformes: Tetraodontidae) Ichthyological Researc 62: 72–113; https://doi.org/10.1007/s10228-014-0428-5

Matsuura, K. (2015). Taxonomy and systematic of tetraodontiform fishes: a review focusing primarily on progress in the period from 1980 to 2014. Ichthyological Research 62: 72–113; http://doi.org/10.1007/s10228-014-0444-5.

Matsuura, K., Golani, D. & S.V. Bogorodsky (2011). The first record of Lagocephalus guentheri Miranda Ribeiro, 1915 from the Red Sea with notes on previous records of L. lunaris (Actinopterygii, Tetraodontiformes, Tetraodontidae). Bulletin of the National Museum of Natural History, Series A 37(3): 163–169.

Mekkawy, I.A. & A.S. Mohammad (2011). Morphometrics and meristics of the three Epinepheline species: Cephalopholis argus (Bloch & Schneider, 1801), Cephalopholis miniata (Forsskal, 1775) and Variola louti (Forsskal, 1775) from the Red Sea, Egypt. Journal of Biological Sciences 11(1): 10–21; 10.3923/jbs.2011.10.21.

Mekkawy, I.A.A., S.A. Saber, S.M.A Shehata & A.G.M. Osman (2002). Morphometrics and meristics of four fish species of genus Epinephelus (Family Seranidae) from the Red Sea, Egypt. Bulletin of Faculty of Science Assiut University 31(1–E): 21–41.

Meng, H.J. & M. Stocker (1984). An evaluation of morphometrics and meristics for stock separation of Pacific herring (Clupea harengus pallasi). Canadian Journal of Fisheries and Aquatic Sciences 41(3): 414–422; https://doi.org/10.1139/f84-049

Meyer, A. (1990). Morphometrics and allometry in the trophically polymorphic cichlid fish, Cichlasoma citrinellum: alternative adaptations and ontogenetic changes in shape. Journal of Zoology 221(2): 237–260; https://doi.org/10.1111/j.1469-7998.1990.tb03994.x

Miranda Ribeiro A de (1913-15). Fauna brasiliense. Peixes. Tomo V. Museu Nacional, Rio de Janeiro.

Monaliza, M.D. & M. Samsur (2011). Toxicity and Toxin Properties Study of puffer fish collected from Sabah Waters. Health and Environment Journal 2(1): 14–17.

Murta, A.G. (2000). Morphological variation of horse mackerel (Trachurus trachurus) in the Iberian and north African Atlantic: implications for stock identification. ICES Journal of Marine Science 57: 1240–1248; https://doi.org/10.1006/jmsc.2000.0810

Mwita, C.J. (2015). Morphometric relationships among the clariid fishes of the Lake Victoria Basin, Tanzania. Open Journal of Marine Science 5(1): 26–32; https://doi.org/10.4236/ojms.2015.51003

Nelson, J.S., T.C. Grande & M.V.H. Wilson (2016). Fishes of the World. Fifth Edition. John Wiley & Sons, Inc., Hoboken, New Jersey: [i]-xli, 1-707; https://doi.org/10.1002/9781119174844

Pierce, G.J., L.C. Hastie, A. Guerra, R.S. Thorpe, F.G. Howard & P.R. Boyle (1994). Morphometric variation in Loligo forbesi and Loligo vulgaris: regional, seasonal, sex, maturity and worker differences. Fisheries Research 21(1–2): 127–148.

Randall, J.E. (1985). On the validity of the tetraodontid fish Arothron manilensis (Proce). Japanese Journal of Ichthyology 32(3): 347–354.

Randall, J.E., S.V. Bogorodsky & J.M. Rose (2012). Colour variation of the puffer Arothron hispidus (Linnaeus) and comparison with A. reticularis (Bloch & Schneider). International Journal of Aquatic Biology 18(1): 41–55.

Regan, C.T. (1903). On the classification of the fishes of the suborder Plectognathi; with notes and description of new species from specimens in the British Museum Collection. Proceedings of Zoological Society of London 1902(2): 284–303.

Reist, J.D. (1985). An empirical evaluation of several univariate methods that adjust for size variation in morphometric data. Canadian Journal of Zoology 63: 1429–1439.

Richardson (1845). Richardson J (1845) Ichthyology.—Part 3. In: Hinds RB (ed) The zoology of the voyage of H.M.S. Sulphur, under the command of Captain Sir Edward Belcher, R.N., C.B., F.R.G.S., etc., during the years 1836-42, No. 10. Smith, Elder & Co, London, pp 71–98, pls 55–64

SAS (2014). SAS User’s Guide, Statistics. SAS Institute, Inc., Cary, NC.

Simon, K.D., Y. Bakar, A. Samat, C.C. Zaidi, A. Aziz & A.G. Mazlan (2010). Population growth, trophic level, and reproductive biology of two congeneric archer fishes (Toxotes chatareus Hamilton, 1822 and Toxotes jaculatrix Pallas, 1767) inhabiting Malaysian coastal waters. Journal of Zhejiang University SCIENCE B 10(12): 902–911; https://doi.org/10.1631/jzus.B0920173

Slábová, M. & D. Frynta (2007). Morphometric variation in nearly unstudied populations of the most studied mammal: the non-commensa house mouse (Musmusculus domesticus) in the near east and northern Africa. Zoologischer Anzeiger 246(2): 91–101; https://doi.org/10.1016/j.jcz.2007.02.003

Smith, J.L.B. (1958). Tetraodont fishes from from south and east Africa. Annals & Magazine of Natural History, Series 13 1: 156–160.

Smith, M.M. (1986). Family No. 267: Tetraodontidae. In: Smith MM, Heemstra PC (eds) Smiths’ sea fishes. Macmillan South Africa, Johannesburg, pp 894-903, pls 139-140, 142-145.

Smith, M.M. & P.C. Heemstra (1986). Family No. 263: Balistidae. In: Smith MM, Heemstra PC (eds) Smiths’ sea fishes. Macmillan South Africa, Johannesburg, pp 876-882, pls 136-139.

Strauss, R.E. & C.E. Bond (1990). Taxonomic methods: morphology. Methods for fish biology. American Fisheries Society, Bethesda, Maryland, 109-140.

Tomovic, L. & G. Dzukic (2003). Geographic variability and taxonomy of the nose horned viper, Vipera ammodytes (L. 1758), in the central and eastern parts of the Balkans: a multivariate study. Amphibia-reptilia 24: 359–377.

Tudela, S. (1999). Morphological variability in a Mediterranean, genetically homogeneous population of the European anchovy, Engraulis encrasicolus. Fisheries Research 42(3): 229–243.