Journal of Threatened Taxa |
www.threatenedtaxa.org | 26 July 2022 | 14(7): 21467–21469
ISSN 0974-7907 (Online) | ISSN 0974-7893
(Print)
https://doi.org/10.11609/jott.7891.14.7.21467-21469
#7891 | Received 21 February 2022 | Final
received 25 April 2022 | Finally accepted 29 June 2022
Cetrelia isidiata (Asahina) W.L. Culb. & C.F. Culb. (Parmeliaceae) – an
addition to the Indian lichen biota
Gaurav K. Mishra 1,
Pooja Maurya 2 & Dalip
K. Upreti 3
1,2,3 Lichenology Laboratory, Plant
Diversity Systematics and Herbarium Division, CSIR - National Botanical
Research Institute,
Rana Pratap Marg, Lucknow, Uttar
Pradesh 226001, India.
2 AcSIR- Academy of Scientific &
Innovative Research, Ghaziabad, Uttar Pradesh 201002, India.
1 gmishrak@gmail.com (corresponding
author), 2 pm953516@gmail.com, 3 upretidknbri@gmail.com
Abstract: Cetrelia isidiata (Asahina) W.L. Culb. & C.F. Culb., is characterized by the presence of isidia, pseudocyphellae on thallus, and containing anziaic acid. The species is reported here as an addition
to the Indian lichen biota from Arunachal Pradesh. A detailed description along
with key to isidiate species of the genus known is
provided.
Keywords:
Ascomycetes, biodiversity, lichenized, taxonomy.
Editor: Anonymity
requested. Date of publication:
26 July 2022 (online & print)
Citation: Mishra, G.K., P. Maurya & D.K. Upreti (2022). Cetrelia
isidiata (Asahina) W.L.
Culb. & C.F. Culb. (Parmeliaceae) – an addition to the Indian lichen biota. Journal of Threatened Taxa 14(7): 21467–21469. https://doi.org/10.11609/jott.7891.14.7.21467-21469
Copyright: © Mishra et al. 2022. Creative Commons Attribution 4.0
International License. JoTT allows unrestricted use, reproduction, and
distribution of this article in any medium by providing adequate credit to the
author(s) and the source of publication.
Funding: Council of Scientific
and Industrial Research (CSIR)-OLP101B
& The University Grants Commission for the award of UGC-JRF.
Competing interests: The authors
declare no competing interests.
Acknowledgements: The authors are thankful to the
director of the CSIR-National Botanical Research Institute, Lucknow for
providing laboratory facilities under the project number OLP101. The author P. Maurya would like to thanks to the University Grants
Commission for award of UGC-JRF. The manuscript number is
CSIR-NBRI-MS/2022/02/09.
The genus Cetrelia
W.L. Culb.
& C.F. Culb. (Parmeliaceae)
is represented by 18 species from the world Randlane
et al. (2013), of which 10 species are reported from India (Mishra & Upreti 2015). According to Randlane
& Saag (2004) the isidiate
species of Cetrelia show their restricted
distribution in Asia whereas sorediate species are
found in European and Asian countries. Culberson & Culberson (1968)
provided a monograph on the genera Cetrelia
and clearly mentioned that Cetrelia isidiata might be mistaken from C. pseudolivetorum in colour
spot test as both species produce a pink colour in C
reaction, therefore, thin layer chromatography (TLC) test will be desirable for
the recognition of olivetoric and anziaic
acids. Cetrelia braunsiana
(Müll. Arg.) W.L. Culb.
& C.F. Culb., and C. pseudolivetorum
are isidiate species of Cetrelia
earlier reported from India (Singh & Sinha 2010).
Materials and Methods
The present study is based on the
Cetrelia specimen preserved in the herbarium
of CSIR-National Botanical Research Institute, Lucknow (LWG). The specimen was
examined morphologically, anatomically, and chemically. Thin hand-cut sections
of thalli were mounted in water or cotton blue and 5% KOH and observed under a
compound microscope. For chemical spot tests the usual reagents of K, C, KC,
and P were used. TLC was performed in solvent system A (Toluene: 1, 4-dioxane:
acetic acid: 180: 60: 8 ml), following the technique of Orange et al. (2001).
The specimen was identified up to the species level with the help of
publications of Mishra & Upreti (2015) and
Culberson & Culberson (1968).
Result
Cetrelia isidiata
was reported
earlier from China, Japan, and Taiwan (Randlane &
Saag 2004). It is a new record for Indian lichen
biota recorded for the first time in Arunachal Pradesh. A detailed taxonomic
description of the species is provided together with illustration, key to the isidiate species and comparative characteristic features of
Indian isidate species of the genus Cetrelia (Table 1).
Cetrelia isidiata (Asahina)
W.L. Culb. & C.F. Culb.
(Image 1, Figure 1)
Contr. U. S. Natl. Herb. 34: 510
(1968).
= Cetrelia
sanguinea Schaer. f. isidiata Asahina, Nov. Fl.
Jap. 5: 73 (1939).
Thallus
foliose, corticolous, loosely attached to the
substratum, 5–19 cm across; lobes 0.5–1.5 cm broad; upper surface grayish or
light brownish, pseudocyphellate; pseudocyphellae
tiny and infrequent; isidia present on mostly margin of lobes, simple, globose
or sometime coralloid or poorly developed; lower surface black, margins brown
or concolorous to upper surface; rhizines black;
medulla white. Apothecia and pycnidia not seen.
Chemistry: Medulla K–, C+ pink or
red, KC–, P−; anziaic acid as major compound,
±atranorin.
Remarks: Cetrelia
isidiata morphologically exhibits its
similarity with C. braunsiana and C. pseudolivetorum but differs in presence of anziaic acid in the thallus. The species is also close to C. sanguinea (Schaer.)
W.L. Culb. & C.F. Culb.,
in having anziaic
acid in the thallus but differs by lacking isidia. In India, the species is
found growing on bark of trees at an elevation of 2,966 m in Eastern Himalayan
state of Arunachal Pradesh.
Specimen examined: 15-037820
(LWG), 16.vii.2015, India: Arunachal Pradesh, Tawang
district, around monastery, on bark, 27.585N, 91.857E, 2,966 m, coll. R.
Bajpai.
A key to the isidiate
species of Cetrelia
Medulla C+ red or pink and thallus containing olivetoric or anziaic acids
….….….…............…. 2
1a. Medulla C-
and thallus containing alectoronic and α-collatolic acids ………...…… C. braunsiana
Isidia poorly developed and anziaic acid present
in the thallus …….. C. isidiata
2a. Isidia well
developed and olivetoric acid present in the thallus
……. C. pseudolivetorum
Table 1. Comparative
characteristic features of Indian isidate species of
the genus Cetrelia.
|
Characteristics |
Name of the species |
||
|
Cetrelia braunsiana |
Cetrelia isidiata |
Cetrelia pseudolivetorum |
|
|
Thallus size |
8–12 cm across |
5–19 cm across |
5–15 cm across |
|
Lobes |
5–15 mm wide |
0.5–1.5 cm wide |
0.5–1.5 cm wide |
|
Upper surface |
Gray or ashy-white |
Grayish or light brownish |
Grayish or grayish-white or
uniformly light brownish or tan in old herbarium specimens |
|
Isidia |
Simple, marginal to sometimes
laminal; often coralloid |
Mostly on margin of lobes;
simple, globose or sometime coralloid or poorly developed |
Along margin and on surface;
simple or coralloid, turning into dorsiventral dissected lobules |
|
Pseudocyphellae |
Punctiform to irregular, rarely
more than 1 mm broad |
Tiny and infrequent |
Punctiform or slightly elongate |
|
Lower surface |
Brown to grayish, the margins
brown or grayish like the colour of the upper
surface |
Black, margins brown or
concolorous to upper surface |
Black, margins brown or
concolorous to upper surface |
|
Apothecia |
Rare, submarginal,
perforate, about 0.5 mm broad, asci 8 spored,
ascospores ovoid, 12–15 × 8–9 μm. |
Absent |
Absent |
|
Pycnidia |
Rare, marginal, black,
pruinose; conidia 1 × 4–6 μm,
rod-shaped |
Absent |
Absent |
|
Spot test |
Medulla K–, C–, KC+ pink, PD– |
Medulla K–, C+ pink or red,
KC–, P− |
Medulla K–, C+ pink or red, KC–
or KC+ pink to red, P− |
|
Chemistry |
Alectoronic and α-collatolic acids (as major substance), ±atranorin. |
Anziaic acid (as major
compound), ±atranorin |
Olivetoric (acid as major
compound), ±atranorin. |
|
Distribution in India |
Himachal Pradesh, Sikkim,
Uttarakhand and West Bengal |
Arunachal Pradesh |
Himachal Pradesh, Sikkim,
Uttarakhand and West Bengal |
For figure &
image - - click here (for full PDF)
References
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Smithsonian Institution Press, Washington.
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& D.K. Upreti (2015). The lichen genus
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236(3): 201–214.
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