Journal of Threatened Taxa |
www.threatenedtaxa.org | 26 August 2021 | 13(9): 19367–19369
ISSN 0974-7907 (Online) | ISSN 0974-7893
(Print)
https://doi.org/10.11609/jott.7185.13.9.19367-19369
#7185 | Received 11 February 2021 | Final
received 09 July 2021 | Finally accepted 21 July 2021
SMALL WILD CATS SPECIAL SERIES
Chemical immobilisation of a
Eurasian Lynx Lynx lynx
(Linnaeus, 1758) (Mammalia: Carnivora: Felidae) with ketamine-dexmedetomidine
mixture in Ladakh, India
Animesh Talukdar 1 &
Pankaj Raina 2
1,2 Department of Wildlife
Protection, Badamibagh, Skara,
Leh, Ladakh 94101, India.
1 animeshtalukdar@rediffmail.com
(corresponding author), 2 pankaj.acf@live.com
Editor: Angie Appel, Wild Cat Network, Bad
Marienberg, Germany. Date
of publication: 26 August 2021 (online & print)
Citation: Talukdar, A. & P. Raina (2021). Chemical immobilisation of a
Eurasian Lynx Lynx lynx
(Linnaeus, 1758) (Mammalia: Carnivora: Felidae) with ketamine-dexmedetomidine
mixture in Ladakh, India. Journal of Threatened Taxa 13(9): 19367–19369. https://doi.org/10.11609/jott.7185.13.9.19367-19369
Copyright: © Talukdar & Raina 2021. Creative Commons Attribution
4.0 International License. JoTT allows unrestricted use, reproduction, and
distribution of this article in any medium by providing adequate credit to the
author(s) and the source of publication.
Funding: Department
of Wildlife Protection, Leh,
Ladakh.
Competing interests: The authors
declare no competing interests.
Acknowledgements: The authors are grateful to Sajad Hussain Mufti, chief wildlife warden, Department of
Wildlife Protection, Ladakh for the necessary
permission to carry out this rescue operation. We thank Tashi
Tsering, Parvez Ahmad, Rinchen
Tundup for their help in executing the rescue
operation, and residents of Kungyam village for their
help and support. We are grateful to Sunetro Ghosal
and Dimpi A. Patel for their valuable comments on
earlier drafts of this manuscript. We also acknowledge three anonymous
reviewers and Angie Appel for their valuable inputs and comments.
The Eurasian Lynx Lynx lynx is a
species of global conservation importance (Breitenmoser
et al. 2015). It ranges from Europe to eastern Asia, including the alpine
steppe of the Tibetan plateau and northern Himalaya (Namgail
2004). In India, it has been recorded in the Trans-Himalayan region of Ladakh (Namgail 2004; Sharma
& Dutta 2005; Kotia et al. 2011). Habitat loss
and poaching have been identified as the major threats to the Eurasian Lynx in
India (Kotia et al. 2011). However, little
information is available about the species in India, especially regarding its
ecology and conservation status (Namgail 2004).
Various wild animals face threats
and even physical injury due to their interactions with humans and various
anthropogenic activities and sometimes require rescue interventions. Such
interventions are justified for their conservation value and importance for
human-wildlife interaction mitigation (Pyke & Szabo 2018). Rescue and
rehabilitation are treatments designed to facilitate the process of recovery
and subsequent restoration of some or all of the individual’s physical,
sensory, and mental capabilities that were lost due to injury, illness, stress
or disease (Jones 1961).
In this context, chemical immobilisation of individual animal is regarded as safe and
effective as it causes minimal stress (Neilson 1999). In felids, ketamine and
dexmedetomidine have been used as an anaesthetic
agent for chemical immobilisation (Lamberski 2015).
Chemical immobilisation
of a Eurasian Lynx: We report the successful chemical immobilisation
of a female adult Eurasian Lynx (Image 1) whose age was estimated to be five
years with an estimated weight of 25kg. It was trapped inside a traditional
stone structure used to store grass and fodder in Kungyam
village. This village is located at an elevation of 4,180m in Nyoma block of Leh district in Changthang, Union Territory of Ladakh
(Figure 1). Villagers alerted the Department of Wildlife Protection. When the
rescue team reached the village on 29 December 2020, they found the animal
unable to escape from the stone structure. It was obviously under severe
stress, most likely due to confinement to a small area for a long time and
because of human presence. Thus, it was decided to intervene and chemically immobilise the individual so to be able to physically
remove it from the structure and take it to the Rescue and Rehabilitation
Centre in Leh for stabilisation.
The ambient temperature was -10°C at the time of the operation.
Since the structure was located
inside the village, the immediate release of the animal was not explored as it
may have injured humans, become injured and even more stressed in the process.
Initial efforts to capture it in a box trap were not successful. Subsequently,
we attempted to chemically immobilise it using a
remotely administered air pressurised syringe
projector, model JM Syringe projector by Dan Inject. For this, two stones from
the wall of the structure were removed and covered with a capture net made of
cotton to prevent its sudden escape. However, the animal was not visible due to
little light inside the structure, and the remote drug delivery system with
ketamine (50 mg/ml; ANEKET, Neon Laboratories limited, India) and
dexmedetomidine (0.5 mg/ml; DEXDOMITOR, Zoetis, US) mixture failed as we were
unable to locate the animal in the dark. At this stage, it tried to escape
through the hole and became entangled in the net. We placed a blanket on its
head and immobilised it chemically using a
combination of ketamine at 4 mg/kg body weight and dexmedetomidine at 0.02
mg/kg body weight with a 5ml disposable syringe (DISPOVAN; Hindustan syringes
and medical devices Limited, India). The drugs were injected intra-muscularly
in the right quadriceps muscle of the cranial thigh at 12:30h.
After four minutes of
administering the injection, at 12:34h, the animal was deeply sedated. Its
respiration was normal at 10 breaths/minute and eyes open with no palpebral
reflex along with minimal salivation. It did not respond to stimuli such as
pinching between digits and ears, showing excellent immobilisation
and analgesia. It was carried to a transportation cage for transfer to the
Rescue and Rehabilitation Centre in Leh for a
thorough examination and subsequent rehabilitation. Examination did not reveal
any external injury of the animal. Physiological parameters such as heart and
respiratory rate, and body temperature were found normal at 42 beats/minute, 10
breaths/minute and 39°C, respectively, within 15 minutes of induction. Its
actual weight was 20kg using a spring weighing scale. Thus, the actual dose
received by the animal was ketamine at 5 mg/kg body weight and dexmedetomidine
at 0.03 mg/kg body weight. After 30 minutes of injection of the drugs at
13:00h, atepamezole at 0.1 mg/kg body weight was
injected into the left quadriceps muscle as reversal with the help of a 3ml
disposable syringe (DISPOVAN; Hindustan syringes and medical devices Limited,
India). A palpebral reflex was the first sign of recovery, noticed at 13:04h
within four minutes of administering the reversal. The individual exhibited
complete recovery after 30 minutes at 13:30h of administering the reversal
injection. The whole capture time was 60 minutes from administering the
ketamine and dexmedetomidine mixture until complete recovery.
There are no studies on chemical immobilisation of the Eurasian Lynx in Ladakh.
The combination of ketamine and dexmedetomidine used in this incidence was
found to be effective at a dose of 5 mg/kg body weight for ketamine and 0.03
mg/kg body weight for dexmedetomidine. The same drug mixture can be
administered remotely. The drug dose used in this instance was similar to the
combinations of ketamine and dexmedetomidine recommended by Schöne
et al. (2002) for the Eurasian Lynx and by Lamberski
(2015) for felids in general.
The drug combination in our
rescue operation was used for the first time on a Eurasian Lynx in India. Our
experience with this combination on a single individual does therefore not
account for possible effects of differences in age and sex of individuals, or
of variations in weather conditions. We recommend to reduce stress in future
rescue operations by minimizing the presence of humans. We propose to develop a
protocol for chemical immobilisations used in rescue
and release operations based on a larger sample size.
For
figure & image - - click here
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