Pethia lutea, a new species of
barb (Teleostei: Cyprinidae) and new records of P. punctata from
northern Western Ghats of India
Unmesh Katwate 1, Chetana Katwate 2, Rajeev
Raghavan 3, Mandar S. Paingankar 4 & Neelesh
Dahanukar 5
1,2 Bombay Natural History Society Hornbill
House, Opp. Lion Gate, Shaheed Bhagat Singh Road, Mumbai, Maharashtra 400001,
India
3 Conservation Research Group (CRG), St.
Albert’s College, Banerji Road, Kochi, Kerala 682018, India
3,4,5 Systematics, Ecology & Conservation
Laboratory, Zoo Outreach Organization (ZOO), 96 Kumudham Nagar, Vilankurichi
Road, Coimbatore, Tamil Nadu 641035, India
5 Indian institute of Science Education and
Research, Dr. Homi Bhabha Road, Pashan, Pune, Maharashtra 411008, India
1 theunmesh@gmail.com, 2 chetanak72@gmail.com,3 rajeevraq@hotmail.com, 4 mandarpaingankar@gmail.com, 5n.dahanukar@iiserpune.ac.in (corresponding author)
Abstract: A new species of barb Pethia luteais described from the Kundalika River in the northern part of the Western
Ghats. The new species can be
distinguished from its congeners in India based on a combination of characters
including a distinct humped nape, absence of barbels, complete lateral line,
lips thick, lateral fold on snout, 19–22 lateral line scales, 8 predorsal
scales, 9–10 prepelvic scales, 14–15 preanal scales,
4–4½ transverse scale rows between lateral line and dorsal fin
origin, 2½–3 transverse scale rows between lateral line and pelvic
fin base, 6–9 pair of serrae on the distal half of the dorsal fin spine,
13–15 branched pectoral fin rays, 7 branched pelvic fin rays, 4+26 total
vertebrae, 4+13 abdominal and 13 caudal vertebrae, body with one vertical
humeral and one caudal blotch and dorsal fin without any bands or blotches.
Additionally, we provide new records of Pethia punctata from the rivers
of Maharashtra State along with a description of its osteology.
Keywords: Conservation status, distribution,
freshwater fish, Maharashtra, taxonomy.
doi: http://dx.doi.org/10.11609/JoTT.o3929.5797-818 | ZooBank: urn:lsid:zoobank.org:pub:27215C3C-B61E-4001-8234-91C00EFDA743
Editor: Anonymity requested. Date
of publication: 26 June 2014 (online & print)
Manuscript details: Ms # o3929 | Received 29
January 2014 | Final received 18 May 2014 | Finally accepted 02 June 2014
Citation: Katwate, U., C. Katwate,
R. Raghavan, M.S. Paingankar & N. Dahanukar (2014). Pethia
lutea, a new species of barb (Teleostei:
Cyprinidae) and new records of P. punctata from northern Western
Ghats of India. Journal
of Threatened Taxa 6(6): 5797–5818; http://dx.doi.org/10.11609/JoTT.o3929.5797-818
Copyright: © Katwate et al. 2014. Creative
Commons Attribution 4.0 International License. JoTT allows unrestricted
use of this article in any medium, reproduction and distribution by providing
adequate credit to the authors and the source of publication.
Funding: The
study was partially funded by small grants of the Critical Ecosystem
Partnership Fund (CEPF) small grant through the Ashoka Trust for Research in
Ecology and Environment to UK (CEPF-ATREE-WGhats/SGP/WGSG186–BNHS_FISHES)
and partially by DST-INSPIRE Faculty Fellowship to ND. RR thanks the North of
England Zoological Society (NEZS)-Chester Zoo for funding.
Competing Interests: The authors declare no
competing interests. Funding sources had no role in study design, data
collection, results interpretation and manuscript writing.
Author Contribution: UK, CK, RR, MP, ND collected specimens. UK, CK performed the
morphological and osteological studies. MP, ND performed the genetic analysis.
UK, RR, ND studied museum specimens. UK, CK, RR, MP, ND wrote the manuscript.
Author Details: Unmesh Katwate is a
Zoology (Oceanography) postgraduate currently working as Scientist-A in Bombay
Natural History Society (BNHS). He works on conservation, taxonomy and
ecological aspects of freshwater fishes and amphibians of the Western Ghats. Chetana
Katwate is a Research Associate in BNHS. She works on freshwater fish
osteology and is interested in understanding its evolutionary significance. She
is also interested in ecology and conservation. Rajeev
Raghavan is interested in interdisciplinary research focused on
generating information and developing methods to support conservation
decision-making in freshwater ecosystems. Mandar S. Paingankar is a
molecular biologist and works on vector biology with an emphasis on host
parasite interactions. He works on molecular phylogeny and systematics of
freshwater fishes as a hobby. Neelesh Dahanukar works in ecology and
evolution with an emphasis on mathematical and statistical analysis. He is also
interested in taxonomy, distribution patterns and molecular phylogeny of
freshwater fish and amphibians.
Acknowledgements: We thank Rajendra Pawar, Rupesh Raut, Vishwas, Abhijit and Saurabh for
helping with the collections; Karsten E. Hartel Curatorial Associate, Museum of
Comparative Zoology, Harvard University, for helpful discussion and providing
image of Francis Day’s material of Puntius punctatus; Asad Rahmani,
Director; Deepak Apte, COO; Rahul Khot, In-Charge Natural History Collection
department, for their help during study of the museum specimens and
registration of specimens in Bombay Natural History Society (BNHS), Mumbai;
Sanjay Molur, Executive Director; and Priyanka Iyer, curator of fish
collection, for holding our specimen vouchers in the museum collection of
Wildlife Information Liaison Development (WILD) Society, Coimbatore; the
Director, Zoological Survey of India and Officer In-Charge of the ZSI
Collections for providing us the access to the type material of the species;
Shrikant Jadhav for his help while studying some of the type material; Ralf
Britz for his help in accessing Day’s material in the collections of the
Natural History Museum, London.
For figures, images, tables -- click here
INTRODUCTION
Genus Pethia is characterized
by small adult size, absence of rostral barbels, maxillary barbels rudimentary
or absent, last unbranched dorsal fin rays osseous and serrated on posterior
edge, 3–4 branched and eight branched dorsal fin rays, three unbranched
and five branched anal fin rays, 11–13 precaudal and 13–16 caudal
vertebrae, complete or incomplete lateral with 19–24 scales in lateral
series—except P. sharmai (Menon & Devi 1993), which has 42
scales—and lateral color pattern consisting of a black blotch on caudal
peduncle with other black blotches, spots or bars often present (Pethiyagoda et
al. 2012; Knight 2013). The genus
is currently known to be endemic to South Asia and Myanmar and comprises 35
species (Pethiyagoda et al. 2012; Knight 2013; Dishma & Vishwanath 2013;
Kottelat 2013; Gurung et al. 2013). In India the genus is represented by 23 species, with seven found in
river systems originating in the Western Ghats.
Pethia ticto(Hamilton, 1822) has long been considered as a widely distributed species found
throughout the Indian subcontinent (Hora et al. 1939; Jayaram 2010). However, recent studies have suggested
that fish previously considered P. ticto represent a complex of several
valid species (Beevi & Ramachandran 2005; Linthoingambi & Vishwanath
2007; Mercy & Jacob 2007; Knight et al. 2012), with P. ticto sensu
stricto possibly restricted to the Ganges and Brahmaputra watershed. Several records of P. ticto from
both east and west flowing rivers in the northern part of the Western Ghats
need taxonomic validations, as they might comprise one or more distinct
species.
While exploring the diversity of Pethiafrom the Western Ghats of Maharashtra, we came across a species distinctly
different from its congeners, which we describe as Pethia lutea.
MATERIALS AND
METHODS
Study site and
sampling
Fishes were collected from seven
localities in five west flowing river systems, viz., Ulhas, Kal, Kundalika, Savitri
and Shastri, part of the northern Western Ghats in Maharashtra State,
India. The specimens were collected
responsibly and not more than three specimens were collected from each site,
except for the type locality where six specimens were collected. In addition, seven specimens of Pethia
punctata were collected from Gad and Terekhol River systems.
Voucher specimens and museum
abbreviations
Voucher specimens are deposited in
the museum collections of the Bombay Natural History Society (BNHS), Mumbai;
the Wildlife Information Liaison Development (WILD) Society, Coimbatore; the
Zoological Survey of India, Western Regional Center, Pune (ZSI-WRC) and the
Conservation Research Group, St. Albert’s College (CRG-SAC), Kochi. Other material examined are in the
museum collections of the Zoological Survey of India, Kolkata (ZSI-K); Natural History Museum, London (BMNH) and the Museum of
Comparative Zoology, Harward University (MCZ).
Morphological and
morphometric analysis
Measurements were taken point to point using dial calipers to the nearest
0.1mm. Subunits of the body are
presented as percent of standard length (SL), and subunits of the head are
presented as percent of head length (HL). All pored scales were counted when reporting the lateral line
scales. Methods for taking counts and
measurements follow Kullander (2008) and Pethiyagoda et al. (2012).
Osteology
Two specimens, BNHS FWF 79 and BNHS
FWF 88, were cleared and stained following the procedure described by Potthoff
(1984). Osteological nomenclature
follows Conway (2011) and the description of osteology follows Pethiyagoda et
al. (2012) and Dishma & Vishwanath (2013) for easy comparison with other
related taxa. Illustrations were made from images
captured by a digital camera fitted on stereo–zoom light microscope
(Leica S8 APO, USA).
Phylogenetic
analysis
Gills were harvested from proposed
new species (BNHS FWF 78, BNHS FWF 73 and WILD-14-PIS-064), P. punctata(WILD-14-PIS-103, BNHS FWF 89, BNHS FWF 90 and BNHS FWF 91), P. setnai(WILD-13-PIS-043, WILD-13-PIS-046, BNHS FWF 53 and BNHS FWF 54) and P.
phutunio (BNHS FWF 95) and were preserved in absolute Ethanol. DNA extraction, PCR amplification for
cytochrome b (cytb) and cytochrome oxidase subunit I (COI) gene sequences and
sequencing protocols follow Katwate et al. (2013) and Ali et al. (2013). Sequences were analyzed by BLAST tool
(Altschul et al. 1990). All sequences generated as part of the study have been
deposited in GenBank under the accession numbers KJ681103–KJ681117.
We used the cytb gene sequence data
from Katwate et al. (2013), while COI gene sequences for Pethia and
related genera were downloaded from NCBI GenBank (http://www.ncbi.nlm.nih.gov/). GenBank accession numbers for sequences
are provided in respective figures. Gene sequences were aligned using MUSCLE (Edgar 2004). Molecular phylogeny was performed using
the freeware MEGA 6 (Tamura et al. 2013). Best fit model for nucleotide substitution was selected from 24 models
using MEGA 6 (Tamura et al. 2013) based on minimum Bayesian Information
Criterion (BIC) value (Schwarz 1978; Nei & Kumar 2000). Maximum likelihood tree was built based
on the best fit model and reliability of the phylogenetic tree was estimated
using bootstrap values run for 1000 iterations.
RESULTS
Taxonomy
Pethia lutea sp. nov.
(Image 1 and Table 1)
urn:lsid:zoobank.org:act:40594E11-1796-44FD-8822-9CD2CDD5A1A9
Type material
Holotype: BNHS FWF 71, 23.xii.2012, 30.8mm SL,
Bhira (18.4410N & 73.2670E, elevation 50m), Kundalika River,
Raigad District, Maharashtra, India, coll. Unmesh Katwate and Chetana Katwate.
Paratypes
(n = 21): 3 exs., BNHS FWF 72, 78 and 79, 23.xii.2012, 31.0–35.1 mm SL, Bhira
(18.4410N & 73.2670E, 50m), Kundalika River, Raigad
District, Maharashtra, India, coll. Unmesh Katwate and Chetana Katwate; 1
ex., WILD-14-PIS-061,23.xii.2012, 35.0mm SL, Bhira (18.4410N & 73.2670E, 50m), Kundalika River, Raigad District, Maharashtra, India, coll. Unmesh Katwate and
Chetana Katwate; 1 ex., ZSI–WRC–3686, 23.xii.2012, 30.5mm SL, Bhira (18.4410N & 73.2670E, 50m), Kundalika River, Raigad
District, Maharashtra, India, coll. Unmesh Katwate and Chetana Katwate;
3 exs., BNHS FWF 73,
80 and 81, 23.vi.2012, 33.2–39.0 mm SL, Karjat
(18.9220N & 73.3320E, 48m), Ulhas River, Raigad District,
Maharashtra, India, coll. Neelesh Dahanukar and M. Paingankar;
2 exs., BNHS FWF 74
and 82, 05.i.2013, 31.0mm and 31.70mm SL, Mangaon (18.2330N & 73.2560E, 7m), Kal River - tributary of Savitri
River, Raigad District, Maharashtra, India, coll. Unmesh Katwate and Chetana
Katwate; 1 ex., BNHS
FWF 75, 23.ix.2013, 26.2mm SL, Mahad (18.0910N & 73.4660E, 16m), Savitri River, Raigad District, Maharashtra, India, coll. Unmesh Katwate, Chetana Katwate, Rajendra
Pawar and Vishwas Shinde; 1 ex., WILD-14-PIS-062, 23.ix.2013, 22.5mm SL, Mahad (18.0910N & 73.4660E, 16m), Savitri River, Raigad District,
Maharashtra, India, coll. Unmesh Katwate, Chetana
Katwate, Rajendra Pawar and Vishwas Shinde; 1 ex., ZSI-WRC-3687, 23.ix.2013, 23.4mm SL, Mahad (18.0910N & 73.4660E, 16m), Savitri River, Raigad District,
Maharashtra, India, coll. Unmesh Katwate, Chetana Katwate, Rajendra Pawar and
Vishwas Shinde; 1 ex., BNHS FWF 76, 26.xi.2013, 25.2mm SL, Shivathar Ghal
(18.1480N & 73.6190E, 145m), Savitri River, Raigad District,
Maharashtra, India, coll. Unmesh Katwate, Chetana Katwate, Rajendra Pawar and
Vishwas Shinde; 1 ex., WILD-14-PIS-063, 26.xi.2013, 30.7mm SL,
Shivathar Ghal (18.1480N & 73.6190E, 145m), Savitri River, Raigad District,
Maharashtra, India, coll. Unmesh Katwate, Chetana Katwate, Rajendra Pawar and
Vishwas Shinde; 1 ex., ZSI-WRC-3688, 26.xi.2013, 23.4mm SL, Shivathar Ghal
(18.1480N & 73.6190E, 145m), Savitri River, Raigad
District, Maharashtra, India, coll. Unmesh Katwate, Chetana Katwate, Rajendra
Pawar and Vishwas Shinde; 2 exs., BNHS FWF 83 and 84, 27.xi.2013, 23.4mm and 21.5mm SL,
Poladpur (17.9830N & 73.4700E, 34m), Savitri River, Raigad District, Maharashtra, India, coll. Unmesh Katwate and Chetana Katwate;
2 exs., BNHS FWF 77
and 85, 16.ix.2013, 26.7mm and 21.9mm SL,
Sangameshwar (17.1870N & 73.5500E, 12m), Shastri River, Ratnagiri
District, Maharashtra, India, coll. Unmesh Katwate and Saurabh Rane; 1 ex., WILD-14-PIS-064, 16.ix.2013, 20.9mm SL, Sangameshwar
(17.1870N & 73.5500E, 12m), Shastri River, Ratnagiri
District, Maharashtra, India, coll. Unmesh Katwate and Saurabh Rane.
Diagnosis
Pethia lutea sp. nov. can be distinguished from its
congeners based on a combination of prominent characters including a distinct
humped nape; complete lateral line; absence of barbels; lips fleshy; distinct
lateral fold on snout; 19–22 pored lateral line scales; eight predorsal
scales; 9–10 prepelvic scales; 14–15 preanal scales;
4½–4 scales between dorsal fin origin and lateral line, and
2½–3 scale between lateral line and pelvic fin origin; last simple
dorsal fin ray strong and serrated with 6–9 serrae on distal half of
spine whereas 2–4 on apical half of spine; 13–15 branched pectoral
fin rays; seven branched pelvic fin rays; caudal fin with 6–7+6–7
principal rays and 8–9+8–9 branched rays; 5 supraneurals; 6
predorsal neural spine; deep and enlarged infraorbital three; gill rakers three
on epibranchial, one at angle and 14–15 on first ceratobranchial; one
humeral band covering 3rd and 4th lateral line scale and
extends to one scale up and down; one caudal blotch encircling caudal peduncle
dorsally covering 17th–19th lateral line scale;
dorsal fin without any bands and body dark yellow with iridescence on scale.
Description
Morphometric and meristic data of
the holotype and 21 paratypes are provided in Table 1. Photographs of the holotype and
paratypes in live and preserved conditions from different localities are
provided in Images 1, 2 and 4, while, osteological details are provided in
Image 3 and Fig. 1.
Body moderately deep, compressed
laterally; dorsal profile from tip of snout to occiput plain, humped at nape
immediately posterior to occiput, rising gradually up to dorsal-fin origin,
thereafter sloping gradually towards hypural notch. Ventral profile moderately
convex up to posterior end of anal-fin base, sloping gradually towards hypural
notch. Caudal peduncle longer than deep, its length 1.2–1.5 times
its depth.
Head small, laterally
compressed. Snout rounded, smooth,
shorter than eye diameter, with a distinct lateral fold overhanging upper
lip. Mature males
with breeding tubercles on snout, cheek, nape and dorsum. Eyes large,
dorso-laterally positioned, closer to snout tip than end of operculum, its
diameter less than or equal to interorbital width. Mouth small, subterminal, ventrally ‘U’
shaped, gape of mouth not reaching to vertical from anterior margin of
eye. Lips fleshy, lower lip not
interrupted. Barbels absent.
Dorsal fin origin opposite to pelvic
fin origin, slightly closer to caudal fin than to tip of snout, its distal
margin concave, its height more or less equal to head length (82.6–109.8
%HL). Dorsal fin
with three simple and seven branched rays, last simple ray strong, spinous,
weakly serrated posteriorly. Pectoral fin with one simple and 13–15 branched rays, its tip
rounded, reaching almost one or two scales anterior to pelvic-fin origin. Pelvic fin with one simple and seven
branched rays, its tip rounded, not reaching vent. Anal fin with three
simple and five branched rays, its distal margin concave. Caudal fin
deeply forked, with 6–7+6–7 principal rays and 8–9+8–9
branched rays.
Lateral line
complete. Lateral
line originates from opercular joint, rise dorsally till humeral spot,
thereafter decreases till vertical from dorsal fin origin, thereafter runs
along with intercalated scale row till end of hypural notch. Lateral line with
19–22 scales with last scale on caudal peduncle unpunctuated (i.e.,
18–21 pored scales), transverse scales 4–½4 between dorsal
fin origin to lateral line, 2½–3 between lateral line to pelvic
fin base, predorsal scales 8, prepelvic scales 9, preanal scales 14–15,
circumpeduncular scales 12. Prepelvic axillary scale present, its exposed length about
one-third of pelvic fin length.
Osteology
Osteology of paratype BNHS FWF 79
(female) is shown in Image 3. Post–epiphysial fontanelle absent (Fig. 1a); infraorbital three
deep, partially overlapping the cheek and preoperculum (Fig. 1b). Gill rackers simple, acuminate (not
branched or laminate), with 3 rackers on epibranchial, one at angle and
14–15 (n=4) on first ceratobranchial. Four predorsal neural
spines present. Four supra neurals present. First pterygiophore of
dorsal fin inserted between 8th and 9th vertebrae. Weberian apparatus constitutes first
four vertebrae. Predorsal vertebrae including weberian
apparatus 8. Total number of
vertebrae 4+26, with 4+13 abdominal and 13 caudal vertebrae. Caudal fin with six hypurals and one
parhypural, last three caudal vertebrae support caudal fin, free uroneural absent
(Fig. 1c). Last simple ray serrated
posteriorly with 6–9 pairs of serrae on distal half of spine, 2–3
serrae on apical half of spine (Fig. 1d).
Coloration
In life (Image 4): Body bright
yellowish with irridescence on scale, each scale bordered with black
pigmentation. Body with one humeral
spot, sometimes appear like a short vertical band, covers 3rd and 4thlateral line scale, extends to one scale up and down, one caudal blotch
encircles caudal peduncle dorsally which covers 17th–19thlateral line scale. Yellow band encircling caudal blotch anteriorly. Dorsal fin plain,
without any bands. Dorsal fin of breeding male red.Pectoral, pelvic and anal fins saffron to red. Caudal fin colorless
or saffron to red in breeding male. Dorsal, caudal and anal fins colored at distal margin. Iris pale yellow, with saffron inner and
outer edges across upper half of eye. Eyes with middle vertical half black streak. Opercular region studded with minute
black and red spots. Infra orbital
region studded with black spots (Image 4e).
In preservative (Image 1, 2): Body
and fin color patterns fade in preservation except humeral and caudal
spots. Body cream colored with
dorsolateral portion above lateral line deeply pigmented.
Etymology
The specific name ‘lutea’ is
Latin for ‘yellow’ and is named for the characteristic bright yellow colored
body in life. Gender feminine.
Common name
Citron Barb
Distribution
The species is restricted to west
flowing river systems in the northern part of the Western Ghats (between
17–19 0N latitudes) in Maharashtra State, India (Image
5). Currently the species is known
from eight localities in six west flowing river systems, viz.,:Ulhas, Kal, Kundalika, Savitri, Jagbudi and Shastri. Extensive surveys have failed to record
this species north of Ulhas River system and south of Shastri River system as
well as east flowing rivers in the northern parts of Western Ghats.
Habitat
Habitat at type locality is shown in
Image 6. The new species was
recorded from riffles and runs with boulders and gravels as substratum. Adult specimens were mostly found to be
associated with submerged vegetation. The species was found only in clear unpolluted river stretches with well oxygenated waters and were not observed in pools and
ditches. Co–occurring species
included those within the genera Salmostoma, Devario, Dawkinsia,Garra, Puntius, Systomus and Anguilla.
Phylogeny
Model test suggested best fit nucleotide substitution model to be Tamura &
Nei (1993) model with gamma distribution and invariant sites (TN93+G+I, BIC =
16146.96, lnL = -7217.72, I = 0.43, G = 0.91) for cytb gene as well as for COI
gene (BIC = 12304.55, lnL = -5222.09, I = 0.52, G = 0.91). Pethia luteasp. nov. was nested within
the clade of Pethia (Fig. 2) conforming its generic status. While P.
lutea sp. nov. wasgenetically distinct from other Pethia species for which genetic data
was available (Fig. 2, 3), specimens of P. lutea sp. nov. from Sangameshwar (southern most distribution limit) and
from Ulhas River at Karjat (northern most distribution limit) were genetically
similar to the topotypic material from Kundalika (Fig. 3).
New records ofPethia punctata
We recorded Pethia punctatafrom Terekhol River at Madkhol (15.94⁰N & 73.91⁰E) and Gad River near Bandiwade (16.15⁰N & 73.55⁰E) thereby extending the
distributional range of this species northwards by about 550km (Image 7). The identity of the species as P.
punctata was conformed from morphology (Images 8,9, Table 2) and genetic
data (COI gene sequence HE801573) of topotypic material (Fig. 3). Pethia punctata was collected
from slowly flowing secondary streams (Image 10) with riparian cover. Stream bed was
sandy with gravel and submerged vegetation (Cobomba sp.). Other co-occurring species in this
locality included those within the genera Horabagrus, Mystus, Aplocheilus,Devario, Rasbora, Puntius, Dawkinsia and Ompok.
One specimen collected from
Bandiwade which was cleared and stained (Image 11)
showed the following osteological characters: Post-epiphysial fontanelle absent
(Fig. 4a); infraorbital 3 moderately deep, partially overlapping the cheek and
preoperculum (Fig. 4b). Gill
rackers simple, acuminate (not branched or laminate), with 3 rackers on
epibranchial, one at angle and 9–10 (n=2) on first ceratobranchial. Four predorsal neural
spines present. Four supra
neurals present. First
pterygiophore of dorsal fin inserted between 8th and 9thvertebrae. Weberian apparatus
constitutes first four vertebrae. Predorsal vertebrae including weberian apparatus 8. Total number of vertebrae 4+25. Caudal fin with six hypurals and one
parhypural, last three caudal vertebrae support caudal fin, paired haemal
spines and neural spines on third preural centra, free uroneural absent, last
neural spine on compound centrum stunted (Fig. 4c). Last simple ray of
dorsal fin strong, spinous, densely serrated posteriorly with 11–14 pairs
of serrae (n=7) on distal half of spine, 2 serrae on apical half of spine (Fig.
4d).
DISCUSSION
Of the 35 valid species of genus Pethia,
only seven occur in the river drainages originating in the Western Ghats
region, viz.,: P. muvattupuzhaensis (Beevi &Ramachandran, 2005), P. narayani (Hora, 1937), P.
nigripinna (Knight, Rema Devi, Indra & Arunachalam, 2012), P. pookodensis (Mercy & Jacob, 2007), P.
punctata (Day, 1865), P. setnai (Chhapgar & Sane, 1992) and P.
sharmai (Menon & Devi 1993). Out of these, only four species, P.
muvattupuzhaensis, P. narayani, P. punctata and P. setnaihave complete lateral line, similar to P. lutea sp. nov., while the other species have an incomplete lateral line.
Pethia lutea sp. nov. can be distinguished
from closely related species that occur in the Western Ghats, having complete
lateral line, based on 19–22 pored lateral line scales (vs. 23–24
in P. punctata and 24–25 in P. muvattupuzhaensis),
6–9 pair of serrae on distal half of last unbranched ray of dorsal fin
(vs. 11–14 in P. punctata and 10–16 in P. setnai) and
13–15 branched pectoral fin rays (vs. 9–10 in P. punctata). Pethia lutea sp. nov. can also be distinguished fromP. punctata and P. setnai in having gill rakers three on
epibranchial, one at angle and 14–15 on first ceratobranchial (vs. three
on epibranchial, one at angle and 8–9 on first ceratobranchial in P.
punctata) and infraorbital three much deep and enlarged (vs. considerable
small and shallow in P. punctata and P. setnai). Pethia lutea sp.nov. has a distinctly
different color pattern with a large humeral spot covering 3rd to 4thlateral line scale which spread over one scale above and below the lateral line
(vs. small humeral spot on 4th–5th scale below the
lateral line in P. punctata and P. muvattupuzhaensis and a
dorsolateral vertical band covering 3rd and 4th lateral
line scales and scales above them in P. setnai) and a caudal blotch
covering 17th to 19th lateral line scales (vs. 19thto 21st in P. punctata and P. muvattupuzhaensisand a vertical transverse band on 16th to 18th lateral
line scales and scales above and below them in P. setnai). Pethia setnai also have a middle
grey band below the dorsal fin (vs. absent in P. lutea sp. nov.) have
distinct eye color pattern in comparison with P. punctata and P.
setnai (Fig. 5). The iris of Pethia
lutea sp. nov. isiridescent yellowish in color with saffron edges and dark mid streak spread
only in upper half of the eye vs. iris dark yellow in color having mid vertical
streak completely spread across mid of the eye in P. punctata and iris
silver in color with yellow hallow around the pupil and radial half streak on
the upper half of the iris in P. setnai (Fig. 5). Pethia lutea sp. nov. differs from P. narayaniby two most prominent characters of having last unbranched ray string and
serrated (vs. feeble, articulated and smooth) and dorsal fin with 8 branched
rays (vs. 9 branched fin rays).
Pethia lutea sp. nov. is distinguished from other
closely related taxa within the Western Ghats by having complete lateral line
(vs. incomplete lateral line in P. pookodensis and P. nigripinna),number of lateral transverse scale rows,
2½–3 between lateral-line scale row and ventral fin origin (vs.
3½ in P.
pookodensis), seven
branched pelvic fin rays (vs. 8 in P. pookodensis) and gill rakers
14–15 on first ceratobranchial (vs. 6 in P. pookodensis and
5–6 in P. nigripinna). The location of humeral spot and caudal blotch also distinguishes P.
lutea sp. nov. from P.
pookodensis and P. nigripinna. A humeral spot, more like a vertical
band covers 3rd–4th lateral line scale
which spreads across one scale above and below the lateral line in P.
lutea sp. nov. (vs. small on 3rd–4thscale of lateral line in P. pookodensis and P. nigripinna),
second large spot on caudal peduncle appears more like a band and covers 17th–19thscale of lateral line and encircles caudal peduncle dorsally (vs. two spot on
caudal peduncle in P. pookodensis, initial covers 16th–17thlateral line scale and later on 19th–20th scale of
lateral line whereas a large caudal spot appears like a band on 18th–19thscale of lateral line in P. nigripinna), body bright yellowish in color
with iridescence on scale (vs. body iridescent silver in P. pookodensis,
adult male generally have deep red body color) and dorsal, pectoral, ventral
and anal fins saffron to red in adult male (vs. pale yellow in P.
pookodensis and black in P. nigripinna).
Pethia
ticto was considered as a widely distributed species
occurring throughout India, Sri Lanka and Myanmar by Hora et al.(1939). However, the wide variation
in the morphological characters from different populations of P. tictostudied by Hora et al. (1939) suggests that the different populations might
represent several distinct species. We, therefore, consider only the data of P. ticto collected from
Ranigunge (West Bengal) by Hora et al. (1939) as it is
the closest population from the type locality of the species namely
‘southeastern parts of Bengal’ (Hamilton 1822, p. 314). Pethia lutea sp. nov. differs from P. tictofrom Ranigunge (Hora et al. 1939) in having a complete lateral line (vs.
incomplete), presence of humeral and caudal spots (vs. absence) and 8 predorsal
scales (vs. 9–11). Description of P. ticto by Linthoingambi & Vishwanath (2007)
is based on the collections from Bramhaputra River system from Assam, Nagaland
and Manipur, which again is not from the type locality of the species. However, P. lutea sp. nov. differs from P. tictodescription by Linthoingambi & Vishwanath (2007) in having complete lateral
line (vs. incomplete), 8 predorsal scales (vs. 9–10), transverse scale
count 4–4½/1/2½–3 (vs. 5½/1/5½) and
dorsal fin plain (vs. dorsal fin with two rows of black bands). Although the
original description of P. ticto is not in details, P. lutea sp. nov. differs from the original
description of P. ticto based on two very prominent characters, first
presence of complete lateral line (vs. lateral line is scarcely
distinguishable) and second, absence of any bands on the dorsal fin in both
sexes (vs. spotted dorsal fin) (Hamilton 1822). Furthermore, P. lutea sp. nov. is genetically distinct
(Figure 2) from P. ticto collected from near its type locality in West
Bengal (26.850N & 80.950E) with a raw distance of
13.7±2.4 % from JQ795475 and 13.7±2.2% from JQ795476 in cytb gene sequence.
Species similar to Pethia tictoappear in the literature by Hamilton (1822) and McClelland (1839) and taxonomic
validity of these names need to be clarified. In the original description of Cyprinus
titius (now a synonym of Puntius chola), Hamilton (1822) referred to
‘Cyprinus tictis’ from north-east parts of Bengal, a
species similar to but differing from Pethia ticto. Hamilton (1822) mentions “…. I took
no notes, and, therefore, until I recover the drawings, I cannot give this fish
a specific character, although I call it Tictis”. Since this species has not been
described and has no diagnostic characters we consider ‘Cyprinus tictis’ as a
nomen nudum. Similarly, McClelland
(1839) in his description of Pethia ticto, refers to a species ‘Cyprinus
bimaculatus’ based on drawings of Hamilton. However, he suggested that ‘..as it has two black spots on each side, it
should rather have been named quadrimaculatus’. Neither ‘Cyprinus bimaculatus’ nor
‘Cyprinus quadrimaculatus’ has any description or diagnostic characters or type
localities. As a result, we
consider both ‘Cyprinus bimaculatus’ and ‘Cyprinus quadrimaculatus’ also as
nomina nuda.
Pethia stoliczkana,
which also has a complete lateral line, was described from Eastern Myanmar by
Day (1871). Pethia lutea sp.nov. differs from P. stoliczkanabased on the original description in having 19–22 scales along the
lateral line (vs.25) (Day 1871). Linthoingambi & Vishwanath (2007) provided description on P. stoliczkanafrom Chindwin basin, which matches largely with the original description except
in transverse scale count between lateral line and ventral fin origin. While, Linthoingambi & Vishwanath
(2007) provide a value of 5½, Day’s (1871) original description mentions
3½. However, it is essential
to note that in the same communication Day (1871) mentions transverse scale
count as 5/6, (i.e., 6 scales between lateral line and pelvic fin base), which
is a contradiction. Therefore, P.
stoliczkana studied by Linthoingambi & Vishwanath (2007) is likely to
be conspecific with P. stoliczkana sunso stricto. Pethia luteasp. nov. differs from P. stoliczkana studied by Linthoingambi & Vishwanath (2007) in having
½4/1/2½–3 transverse scale count (vs.
½5/1/5½), first pterygiophore of dorsal fin inserted between 7thand 8th vertebra (vs. between 8th and 9thvertebra) and no bands on dorsal fin (vs. two dark bands on dorsal fin).
Jerdon (1849) described Systomus
tripunctatus from the coast of Canara (= southern Karnataka). Current taxonomic status of S. tripunctatusis uncertain because of the vague original description. Pethia lutea sp. nov. can however be distinguished
from S. tripunctatus based on the distinct color pattern in the two
species. Jerdon (1849) mentions three spots over the body, two black spots
under end of the dorsal and one at the base of the tail, a color pattern which is drastically distinct from that of P. lutea sp.
nov.
Pethia lutea sp. nov. can be easily distinguished
from all other Indian congeners from Ganga-Brahmaputra and Chindwin-Irrawaddy,
drainages as well as other rivers in Myanmar, except P. macrogramma (Kullander,
2008), P. tiantian (Kullander & Fang, 2005), P. expletiforisDishma & Vishwanath, 2013 and P. guganio (Hamilton, 1822), by having
a complete lateral line (vs. incomplete lateral line). Pethia lutea sp. nov. differs from P.
expletiforis by having eight predorsal scales (vs. 9 predorsal) and
presence of vertical humeral spot (vs. absence of humeral spot); from P.
guganio by having less number of scales (19–22 in lateral series vs.
greater number of scales, 29–30) and less number of scales in transverse
row (4–½4/1/2½–3 vs. ½5/1/4); from P.
macrogramma by a more wide spread vertical humeral spot on 3rd–4thlateral line scale (vs. very small or negligible on 3rd scale of
lateral line) and less number of transverse row scale
4–½4/1/2½–3 (vs. greater number of scales in
transverse row ½4/1/4½); from P. tiantian by having less
number of principle (6–7+6–7) and more number of branched
(8–9+8) caudal fin rays (vs. more number of principle (9–10+9) and
less number of branched (4–6+5) caudal fin rays).
Pethia lutea sp. nov. also differs from its close
Sri Lankan congeners, P. nigrofasciata (Günther, 1868), which also has a
complete lateral line, by having two spots on flank, one humeral spot and one
caudal (vs. three vertical bands on body), more number of prepelvic
(9–10) and preanal scales (14–15) (vs. 5–6 prepelvic and 11
preanal scales), interorbital 3 deep and more wide (vs. relatively small
interorbital 3); dorsal, pectoral, pelvic and anal fins saffron in color, body
yellowish (vs. smoky grey fins and body crimson red and black). Pethia lutea sp. nov. is also genetically distinct
from P. melanomaculata (Deraniyagala, 1956) (Fig. 2) by a raw distance
of 15.3±2.4%.
Recently, Knight (2013) placed Puntius
sharmai Menon & Devi, 1992 within the genus Pethia. However, in our opinion P. sharmaiis less likely to be congeneric with Pethia and warrants further studies
to understand its exact systematic position. Two other species, Puntius
deccanensis Yazdani & Rao, 1976 and Puntius fraseri Hora &
Misra, 1938, which also possess more than 42 lateral series scales, serrated
last unbranched dorsal fin ray and pair of maxillary barbels similar to P.
sharmai, are less likely to belong to genus Pethia. Based on the
same characters, Pethia lutea sp. nov. differs greatly from Puntius deccanensis and Puntius
fraseri, which are also from the northern part of the Western Ghats.
Pethia lutea sp. nov. is restricted in distribution
to the west flowing river systems of the northern part of Western Ghats
(between 17–19 ⁰N
latitudes) in Maharashtra State, India and extensive surveys have failed to
record the species south of 17⁰N
latitude and north of 190N latitude as well as east flowing rivers
of the region. As a result, the expected extent of occurrence (EOO) is roughly
6000km2 and area of occupancy (AOO) is less than 200km2. The species was found only in fast
flowing and clear and unpolluted river stretches, and even after extensive
surveys was not found in polluted waters, especially near industrial zones near
Mahad and Roha. Therefore,
increasing urbanization and industrialization in this area are likely to affect
the populations of this species adversely. Frequent mass fish kills were observed near Mahad and Roha (U. Katwate,
pers. obv. 2010–2013) during the premonsoon period mainly because of
heavy discharge of industrial effluents. Further, in several locations, including Shivathar Ghal, Walan Kond and
Mahad on Savatri River System and Bhira on Kundalika River, the
habitat of this species is also modified by extensive sand mining. As the species prefers flowing waters,
presence of established and proposed irrigation and hydroelectric projects,
that cut the flow of water and create semi-lacustrine conditions, could be a
plausible threat to the species. Based on these observations we propose a draft IUCN Redlist status for
this species as ‘Endangered’ (Box 1). Fortunately, at least one of the populations from Walan Kond (Savitri
River system) is protected through a community sanctuary maintained by local
indigenous communities (Katwate et al. 2014).
In this study, we also extend the
range of Pethia punctata by a distance of ~550km. Pethia punctata was described by
Day (1865) as Puntius punctatus from the erstwhile Princely State of
Cochin, Kerala, India. The generic
status of the species was later changed to Barbus (Day 1889). Hora et al. (1939) synonymized Puntius
punctatus to Puntius ticto. However, Silas (1952) considered the species to be a valid subspecies P.
ticto punctatus. Even though Talwar & Jhingran (1991) and Jayaram
(1991) considered Puntius punctatus as a synonym of Puntius ticto; Menon (1999) and Devi et al. (2007) considered Puntius
punctatus as a valid species. Recently Pethiyagoda et al. (2012) revised
the generic status of the species to Pethia.
Pethia punctata is
currently known from west and east flowing rivers in the southern part of
Western Ghats in Karnataka, Kerala and Tamil Nadu from Mangai Malai
Kulasekaram, Kanyakumari Wildlife Sanctuary and west flowing rivers of
Kanyakumari District; Mathalamparai, Tirunelveli District; Pookode Lake and
parts of Wayanad; Chalakkudy, Muvattupuzha and Periyar rivers of Ernakulam
District; and the Indira Gandhi Wildlife Sanctuary in the Anamalai Hills of
Tamil Nadu (Shaji & Easa 1995; Devi et al. 2005, 2007; Beevi &
Ramachandran 2009; Johnson & Arunachalam 2009; Knight et al. 2012). Although the species has also been reported
from the east flowing Mula-Mutha River of Pune (Tonapi & Mulherkar 1963),
this record has been considered doubtful (Dahanukar 2011) as the species was
neither reported by earlier (Fraser 1942; Suter 1944) nor later researchers
(Kharat et al. 2001; Wagh & Ghate 2003) in spite of extensive surveys.
Similarly, the report of the species from Sri Lanka (Menon 1999) also needs
taxonomic confirmation (Dahanukar 2011).
Our record of Pethia punctata,
backed up with morphological and genetic data, suggests that the species is
also present in west flowing rivers of southern Maharashtra, namely Terekhol
and Gad. This extends the
distributional range of the species by about 550km in the northern Western
Ghats as the previous northernmost record was from Wyanad in Kerala by Shaji
& Easa (1995). Although, the
species is known from both west flowing and east flowing rivers of the southern
Western Ghats, there is a need for validating the reports from east flowing rivers,
preferably using genetic analysis. Pethia
punctata is currently assessed as ‘Least Concern’ in the IUCN Red List of
Threatened Species (Dahanukar 2011); however, the populations of P. punctatain the localities from where collections were made as part of this study are
threatened by inflow of sewage water and tourism-related pollution. Further, the area is also subjected to
riparian deforestation for mango and cashew plantations. Populations of the species downstream of
rocky outcrops in Bandiwade are threatened by siltation and habitat degradation
by laterite quarrying.
The northern Western Ghats of India
are relatively less explored in terms of their fish diversity and distribution
and this is especially true for the west flowing rivers in the Konkan region
(Dahanukar et al. 2011; Katwate et al. 2012). The description of Pethia luteasp. nov. and range extension
of P. punctata points out that the northern Western Ghats of India is
subject to both the Wallacean (incomplete information regarding the
distribution of species) and Linnean (many species still not formally
described) shortfalls (Bini et al. 2006). The fact that the populations of Pethia lutea sp. nov.are also
highly threatened further suggests that there is an immediate need to focus our
attention towards conservation of freshwater ecosystems and biota of this
region. Because no conservation
action plan will be possible in the absence of information on diversity and
distribution of species, our study suggests that further detailed studies on
the taxonomy, distribution and threats to the habitats and taxa should be
encouraged.
Comparative
material
Pethia punctata(n=11): Day’s material (syntype?), 1 ex., MCZ 4303,
Canara (Cannanore, on the Malabar Coast, Kerala, India), coll. F. Day (only
photograph examined); Day’s material (syntype?), 1 ex., BMNH 1889.2.1.755,
Wayanad, Kerala, India, coll. F. Day (only photograph examined); 3 exs.,
CRGSAC-2010.05.01-03, Cochin, Kerala, collected on 18.v.2010 by F. Baby; 6
exs., BNHS FWF 86–90, 92, Bandiwade, Gad River, Sindhudurg District,
Maharashtra, collected on 15.ix.2013 by U. Katwate and S. Rane; 1 ex. BNHS FWF
91, collected from Terekhol River at Madkhol, Maharashtra, by U. Katwate, M.
Paingankar and N. Dahanukar on 9.viii.2013.
Pethia setnai(n=35): Holotype, ZSI Kolkata FF2766, collected from Sanguem, Goa, by S. R.
Sane on 1.iii.1985; Paratypes, 6 exs., ZSI Kolkata
FF2767, collected from Sanguem, Goa, by S. R. Sane on 1.iii.1985; 9 exs., BNHS
FWF 53, 63 to 70, collected from Sanguem, Goa, by U. Katwate, M. Paingankar and
N. Dahanukar on 10.viii.2013; 3 exs.; WILD-13-PIS-043 to 045, collected from
Sanguem, Goa, by U. Katwate, M. Paingankar and N. Dahanukar on 10.viii.2013; 2
exs., ZSI-WRC-P/3567, collected from Sanguem, Goa, by U. Katwate, M. Paingankar
and N. Dahanukar on 10.viii.2013; 9 exs., BNHS FWF 54–62, collected from
Terekhol River at Madkhol, Maharashtra, by U. Katwate and N. Dahanukar on
12.vi.2013; 3 exs., WILD-13-PIS-046 to 48, collected from Terekhol River at
Madkhol, Maharashtra, by U. Katwate and N. Dahanukar on 12.vi.2013; 2 exs.,
ZSI-WRC-P/3568, collected from Terekhol River at Madkhol, Maharashtra, by U.
Katwate and N. Dahanukar on 12.vi.2013. Osteological details were obtained from
Katwate et al. (2013).
Pethia narayani (n=2): Syntypes, 2 exs.,ZSI Kolkata F12180/1, collected from Cauvery River, Coorg, by C.R.N. Rao (only photographs examined).
Pethia
pookodensis (n=2): 2 exs., specimens not
collected, from Pookode lake, Kerala, by R. Raghavan and A. Ali, on 14.04.2004.
Photographs are provided in Appendix C.
Puntius fraseri(n=3): Holotype (based on the note by R. Pethiyagoda in the specimen bottle),
ZSI-K F12497/1, collected from Dharna River, Deolali, Bombay Presidency by A.G.L.
Fraser; 2 exs., Paratypes, ZSI-K F12497/1, collected
from Dharna River, Deolali, Bombay Presidency by A.G.L. Fraser.
Puntius
deccanensis (n=4): Holotype, ZSI-K FF1925, collected from nalla
near Katraj tank, Poona, by C. B. Prasad on 20.vii.1974; 1 ex.,paratype FF1927, collected from nalla near Katraj tank, Poona, by B.K. Tikader
on 13.ii.1976; 2 exs., paratypes, collected from nalla near Katraj tank, Poona,
by C. B. Prasad on 20.vii.1974.
Pethia phutunio (n=3): 1 ex., BNHS-FWF-95, collected from Sambalpur,
Odisha, by S. Jadhav, on 7 July 2012; 2 exs., BNHS FWF 93 and 94, collected
from Hooghly, West Bengal, by R. Pandit on 12 May 2010.
Pethia muvattupuzhaensis:
Data from Beevi & Ramachandran (2005).
Pethia nigripinna: Data
from Knight et al. (2012).
Pethia
macrogramma: Data from Kullander (2008).
Pethia
stoliczkana: Data from Linthoingambi & Vishwanath (2007)
Pethia tiantian: Data
from Kullander & Fang (2005)
Pethia
expletiforis: Data from Dishma & Vishwanath (2013)
Pethia guganio: Data
from Knight (2013).
Pethia ticto: Data
from Linthoingambi & Vishwanath (2007), Hora et al. (1939), Hamilton
(1822).
Pethia
nigrofasciata: Data from Jayaram (1991) and Pethiyagoda et al.
(2012).
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