Pollination ecology of
the Gray Nicker Caesalpinia crista (Caesalpiniaceae) a mangrove
associate at Coringa Mangrove Forest, Andhra Pradesh, India
P. Suvarna Raju 1& A.J. Solomon Raju 2
1,2 Department of Environmental Sciences,
Andhra University, Visakhapatnam, Andhra Pradesh 530003, India
1 suvarnarajup@rediffmail.com, 2 ajsraju@yahoo.com
(corresponding author)
Abstract: Caesalpinia crista L., commanly known as Gray
Nicker, is an oligohaline mangrove associate confined to landward marginal
areas of the Coringa Mangrove Forest, Andhra Pradesh, India. The flowering occurs during the wet
season from June to November. The flowers are hermaphroditic, self-compatible
and exhibit a mixed breeding system. The floral characteristics that constitute
melittophilous pollination syndrome include diurnal anthesis, slight fragrance,
zygomorphy, yellow petals, with a flag petal displaying a conspicuous nectar
guide, and the presence of nectar with a high sugar concentration. Extra-floral nectar along the rachis is
an additional attractant and is easily perceivable by bees. The plant is pollinated almost
exclusively by bees, especially carpenter bees. The floral characteristics such as free
petals, fully exposed stamens with dry and powdery pollen grains and hairy
stigma facilitate anemophily which is effective due to high winds during the
rainy season. The prolific growth
and near synchronous flowering at population level contribute to pollen
availability in huge quantities and enable anemophily as an effective mode of
pollination. The functionality of
melittophily and anemophily together constitutes ambophily. Hand-pollination experiments indicated
that the plant is principally out-crossing. The natural fruit set does not
exceed 10%; this lowest percentage could be partly due to flower-feeding by the
beetle, Mylabris phalerata. The fruits are indehiscent, 1-seeded, which are buoyant and are not
dispersed far away from the parental sites. The viable seeds produce new plants in
the vicinity of parental plants during the rainy season. This plant builds up
its population as small patches or in pure stands and hence is important in
building landward mangrove cover.
Keywords: Ambophily, Caesalpinia crista, Gray Nicker, mixed
breeding system, self-compatibility.
doi: http://dx.doi.org/10.11609/JoTT.o3754.6345-54
Editor: Cleofas Cervancia, University of Philippines Los
Baños College Laguna, Philippines. Date
of publication: 26 September 2014 (online & print)
Manuscript details: Ms # o3754 | Received 23
August 2013 | Final received 30 August 2014 | Finally accepted 01 September
2014
Citation: Raju, P.S. & A.J.S. Raju (2014). Pollination ecology
of the Gray Nicker Caesalpinia crista (Caesalpiniaceae) a mangrove
associate at Coringa Mangrove Forest, Andhra Pradesh, India. Journal of
Threatened Taxa 6(10): 6345–6354; http://dx.doi.org/10.11609/JoTT.o3754.6345-54
Copyright: © Raju & Raju 2014. Creative Commons Attribution 4.0 International License. JoTT
allows unrestricted use of this article in any medium, reproduction and
distribution by providing adequate credit to the authors and the source of
publication.
Funding: Self-funded.
Competing Interest: The authors declare no
competing interests.
Author Contribution: Both the authors contributed
to a similar extent overall.
Author Details: P. SUVARNA RAJU has been
awarded PhD under Prof. A.J. Solomon Raju. He is currently doing post-doctoral
research. Prof. A.J. Solomon Raju is
Head of the Department of Environmental Sciences, Andhra University,
Visakhapatnam. He is presently working on endemic and endangered plant species
in southern Eastern Ghats forests with financial support from MoEF and CSIR.
Acknowledgements: We thank Andhra University
for providing the necessary administrative help and the State Forest
Department, Government of Andhra Pradesh for permission to work in the Coringa
Mangrove Forest.
For
figures, images, tables -- click here
INTRODUCTION
Caesalpinia is a genus of Caesalpiniaceae family. It is pantropical with 120–150
species of trees, shrubs, and lianas, but the study of the pollination ecology
of the genus is so far limited to the New World species (Lewis 1998). A few species of Caesalpinia have
been studied with regard to their pollination: C. pulcherrima (Ali 1932;
Cruden & Hermann-Parker 1979; Bullock 1985); C. gilliesii (Coccuci
et al. 1992); C. calycina and C. pluviosa var. sanfranciscana(Lewis & Gibbs 1999). Four
flower types occur in Caesalpinia, each of which is adapted to a
different pollinator (bee, butterfly, moth and hummingbird) suggesting that the
floral character suites in the segregated species of Caesalpinia may be
closely related to the type of pollinators (Vogel 1990; Shi-Jin et al. 2004). C. brevifolia, C. coriaria, C.
echinata, C. sappan and C. spinosa are self-compatible, out-crossing
and bee-pollinated (Roubik 1995).
In
the Caesalpinia genus, only two species, namely, C. bonduc (Gray
Nicker) and C. crista (Gray Nicker) extend into beach vegetation and are
frequently found to be mangrove associates; one is pantropical and the other
Asian. C. bonduc is a coarse
scrambling vine, and widely distributed partly because seeds can float and
retain their viability in water for extended times. Its reproductive biology is
not known. C. crista is a
back-mangal spiny climber distributed from India and Ceylon through most of
Southeast Asia to the Ryu-Kyu Islands, Queensland, and New Caledonia (Tomlinson
1986). A study by Shi-Jin et al.(2004) indicated that it is a hermaphroditic species and pollinated by wind and
insects. But, the details of pollination ecology are not available and hence,
the present study was contemplated with the objective of providing floral
biology, breeding system, pollination system, and fruiting aspects of C.
crista in order to understand the ability of this species to survive and
form its population in the landward areas of Coringa mangrove forest in India.
MATERIALS AND METHODS
The
Coringa Mangrove Forest covering an area of 188km2 lies between 16030’–17000’N
and 82010’–80023’E. It is located in the delta in East
Godavari District; it is created by the River Godavari which is 1,330km long
and the second longest river in India. It branches into Vasishta and Gautami near Dowleswaram, which is
considered as the head of the delta. Two distributaries Coringa and Gaderu branching-off the northern bank of
the rivers, Gautami-Godavari, supply freshwater to the Coringa mangroves. Freshwater flows into the mangrove
wetlands of the Godavari delta for a period of six months and peak flow normally
occurs during July to September, coinciding with the southwest monsoon
season. During this period the
entire delta, including the mangrove wetland is submerged under freshwater,
since penetration of sea water is completely blocked by the large amount of
incoming freshwater. Brackish water
condition prevails from October to February and sea water dominates the entire
mangrove wetland from March to May due to the absence of freshwater discharge.
Caesalpinia crista is a prickly climber and is confined to landward localities of
the mangrove forest. It is quite
prominent during its flowering season due to its attractive bright yellow
flowers. It is used in Ayurveda;
the seeds, the leaves and the bark are used in the treatment of amenorrhoea,
dysmenorrhoea, diabetes, intermittent fevers, febrifuge, anthelmintic,
expectorant, as a uterine stimulant and to cleanse the uterus (Jabbar et al.
2007).
Field
experiments were conducted on C. crista during the period from February
2010 to October 2012. Observations regarding the organization of
inflorescences, the spatial positioning of flowers, and their position on the
plant were made since these features are regarded as important for foraging and
effecting pollination by flower-visitors. The flower longevity was recorded by marking twenty just open flowers
and following them until they fell off. Anthesis was initially recorded by observing ten marked mature buds in
the field. Later, the observations were repeated five times on different days,
each day observing twenty marked mature buds in order to provide accurate
anthesis schedule. The same marked
mature buds were followed for recording the time of anther dehiscence. The
presentation pattern of pollen was also investigated by recording how anthers
dehisced and confirmed by observing the anthers under a 10x hand lens. The details of flower morphology such as
flower sex, shape, size, colour, odour, sepals, petals, stamens and ovary were
described.
Twenty
mature but un-dehisced anthers were collected from five randomly chosen plants
and placed in a petri dish. Later, each time a single anther was taken out and
placed on a clean microscope slide (75x25 mm) and dabbed with a needle in a
drop of lactophenol-aniline-blue, the anther tissue was then observed under the
microscope for pollen, if any, and if pollen grains were not there, the tissue
was removed from the slide. The
pollen mass was drawn into a band, and the total number of pollen grains was
counted under a compound microscope (40x objective, 10x eye piece). This procedure was followed for counting
the number of pollen grains in each anther collected. Based on these counts, the mean number
of pollen produced per anther was determined. The mean pollen output per anther
was multiplied by the number of anthers in the flower for obtaining the mean
number of pollen grains per flower. The characteristics of pollen grains were also recorded. The
pollen-ovule ratio was determined by dividing the average number of pollen
grains per flower by the number of ovules per flower. The value thus obtained was taken as
pollen-ovule ratio (Cruden 1977). The stigma receptivity was observed visually and by H2O2 test. In the visual method, the stigma
physical state (wet or dry) and the unfolding of its lobes were considered to
record the commencement of receptivity; withering of the lobes was taken as a
loss of receptivity. H2O2 test as given in Dafni et al. (2005) was
followed for noting stigma receptivity period.
The
presence of nectar was determined by observing fifty mature buds and open
flowers collected at random from 10 plants. Individual volumes of nectar was
recorded for 25 flowers and then the average volume of nectar per flower was
determined and expressed in µl. The
flowers used for this purpose were bagged at mature bud stage, opened after
anthesis and squeezed nectar into micropipette to measure the volume of nectar.
Nectar sugar concentration was also simultaneously determined using a Hand
Sugar Refractometer (Erma, Japan).
Fifty
flowers each from 10 randomly selected plants were used for each mode of
breeding system. The flowers were
emasculated prior to anther dehiscence and bagged for fruit set through
apomixis. The stigmas were pollinated with the pollen of the same flower
manually by using a brush; they were bagged for fruit set through manipulated
autogamy. The flowers were
fine-mesh bagged without hand pollination for fruit set through spontaneous
autogamy. The emasculated flowers
were hand-pollinated with the pollen of a different flower on the same plant;
they were bagged and followed for fruit set through geitonogamy. The emasculated flowers were pollinated
with the pollen of a different individual plant and bagged for fruit set
through xenogamy. All these modes
of pollination were followed for one month for calculating the percentage of
fruit set in each mode. Twenty
inflorescences consisting of 200 flowers were tagged on 20 plants prior to
anthesis and followed for fruit and seed set rate in open-pollinations. Fruit maturation period, fruit dehiscence,
seed dispersal and establishment were observed in detail.
The
insects visiting the flowers were bees and a butterfly. They were observed carefully for 10
hours in a day for 15 days in different weeks during the flowering season. The hourly foraging visits of each bee
species were recorded on 10 different days for which 30 inflorescences were
selected. The data obtained was
used to calculate the percentage of foraging visits made by each bee species
per day and also to calculate the percentage of foraging visits of each
category of bees per day in order to understand the relative importance of each
bee species. Simultaneously, the
bees were observed for their foraging behavior such as mode of approach,
landing, probing behaviour, the type of forage they collect, contact with
essential organs to result in pollination and inter-plant foraging
activity. The bees were captured
from the flowers from 1000–1200 hr on five different days for pollen analysis
in the laboratory. For each bee
species, 10 specimens were captured and each specimen was washed first in ethyl
alcohol and the contents stained with aniline-blue on a glass slide and
observed under a microscope to count the number of pollen grains present. In case of pollen collecting bees, pollen
loads on their corbiculae were separated prior to washing them. From pollen counts, the average number
of pollen grains carried by each bee species was calculated to know the pollen
carryover efficiency of different bees. A beetle was found to be the flower predator. A sample of 500 flowers collected from
75 inflorescences selected at random was used for calculating the flower
predation rate by this beetle.
Plant,
flower and fruit details together with insect foraging activity on flowers were
photographed with a Nikon D40X Digital SLR (10.1 pixel) and a TZ240 Stereo Zoom
Microscope with SP-350 Olympus Digital Camera (8.1 pixel). Magnus Compound
Microscope - 5x, 10x, 40x and 100x magnification was used for studying pollen
characteristics.
RESULTS
Phenology
It
is a large climber with glossy branchlets more or less armed with recurved
prickles. It is a mangrove
associate and characteristically restricted to the landward side. The flowering occurs en masse from June
to November (Image 1a). An individual
plant flowers for about three months. The flowers are produced in racemes which arise from leaf axils and also
aggregated into terminal inflorescences. A raceme produces 33.26±6.1 (Range 27–42) flowers over a period of
about ten days. Flower buds mature
and open in an acropetal manner (Image 1b,c). The plants begin leaf shedding during
late winter and stays leafless until the new leaf flushing which occurs after
the summer showers in May.
The flower
Flowers
are pedicellate, gullet-shaped, small, 15mm long, 10mm wide, yellow, slightly
fragrant, bisexual and zygomorphic. Calyx has five sepals, greenish yellow, 5mm long, basally fleshy, thick
and flattened, terminally curved into a boat-like structure and glabrous. The
corolla is a bright yellow with five petals; the posterior petal is flag or
banner-like with reddish nectar guide, flanked by two wing petals and two
anterior petals. They are clawed,
imbricate, 6mm long, 4mm wide and distinct. Stamens are 10, free, 8mm long,
filaments unequal, hairy and form a white and pubescent 12mm long pseudotube at
the base. The anthers are bilobed,
light yellow, 1mm long, exserted, introrse and versatile. Ovary is 1-carpelled with one locule and
one ovule on marginal placentation. The style is 11mm long, light green, glabrous with simple stigma, the
rim of which is ciliated and chambered.
Floral biology
The
mature buds open from 0600–1100 hr by the unfolding of petal lobes. Petals expand horizontally but do not
reflex; then the stamens, style and stigma are exposed (Image 1d–f). The style and stigma extend beyond the
stamens (Image 1g). The anthers
dehisce by longitudinal slits at anthesis. The pollen output per anther is
654±42.7 (Range 598–726) and per flower, it comes to 6540±420.7. Pollen grains are large, yellow,
prolate, spheroidal, trizonocolporate, powdery and 49.8µm in size (Image
1i). The pollen-ovule ratio is
6,540:1. The stigma attains receptivity two hours after anther dehiscence;
stigma is wet during the receptive period which extends until the evening of
the second day (Image 1h). A flower
produces 1.35±0.2 (Range 0.6–3.1) µl of nectar at the flower base and its
access is restricted to the openings between the bases of the three upper stamens.
The nectar sugar concentration is 30±2.35% (Range 20–34 %). Extra-floral nectaries appear along the
rachis and the nectar continues to secrete even after the flowers wither. The flowers fall off after 4–5
days in un-pollinated ones while the ovary remains and grows by gradual bulging
to produce fruit in pollinated ones.
Breeding systems
The
results of breeding systems indicate that the flowers are self-compatible and
self-pollinating. Fruit set is 6%
in autogamy (unmanipulated) 16% in autogamy (manipulated), 34% in geitonogamy
while it is 62% in xenogamy and 9.5% in open-pollinations (Table 1).
Pollination and Pollinators
The
flowers are unspecialized and the stamens and stigma become exposed when the
petals expand horizontally. They
were foraged on during day time consistently from 0700–1700 hr. The foragers included bees, Apis
dorsata (Image 1j), Pithitis binghami, Nomia sp., Amegillasp. (Image 1k), Xylocopa pubescens (Fig. 1l), X. latipes (Image
2a) and Xylocopa sp. (Image 2b), Ceratina sp. (Image 2c), and the
butterfly, Catopsilia Pomona (Image 2d). Of these, Xylocopa species and C.
pomona collected only nectar while the other bees collected both nectar and
pollen. Their foraging activity
gradually increased from morning to evening with peak activity around noon time
(Fig. 1). Of the total foraging
visits made by both bees and the butterfly, A. dorsata and Ceratina sp.
each made 15%, P. binghami, X. pubescens and Amegilla sp. each
12%, X. latipes 10%, Xylocopa sp. 9%, Nomia sp. 7% and C.
pomona 8% (Fig. 2). Apis, Xylocopa and Amegilla bees were found to depress efficiently the
anterior petals to access nectar while Pithitis and Nomia were
found to have easy access to the nectar in flowers previously foraged by Xylocopaand Amegilla. However, Pithitis, Nomia and Ceratinabees were found to collect pollen easily as the anthers are well exposed. Apis, Xylocopa and Amegilla while
probing the flower for nectar and/or pollen invariably contacted the stamens
and stigma. The other bees also
collected both pollen and nectar but primarily concentrated on pollen
collection during which their ventral side established contact with the stigma.
Body washings of bees revealed the presence of pollen grains; the mean number
varied from 123 to 494 (Table 2). The collection of nectar by bees led to the gradual reduction in the
standing crop of nectar and hence they were compelled to visit as many flowers
as possible from several plants during the day. The inter-plant activity was more during
the afternoon period. Their foraging activity was found to be effecting both
self- and cross-pollination. The
butterfly, C. pomona is not a consistent forager throughout the
flowering season while the bees were consistent in their foraging activity
throughout the flowering season. The butterfly mostly probed the flowers by stretching out the proboscis
to the flower base to collect nectar during which it occasionally contacted the
stamens and stigma and hence was not important as a pollinator. The nectar collecting bees also visited
the extra-floral nectaries for nectar collection and this appeared to promote
their foraging rate.
The
locations of C. crista are windy during the rainy season during which
flowering occurs. The rainy season
is normally associated with gale or cyclonic winds. The pollen being dry and
powdery especially on clear sunny days is exposed to the ambient air. The pollen is blown away by the wind on
such days and it can be seen on the leaves; hence wind is also considered to be
playing a role in effecting self- and cross-pollination.
Flower predation
Further,
a beetle, Mylabris phalerata was found to feed on floral parts including
stamens and stigma (Image 2e); its flower-feeding behaviour was considered to
be affecting reproductive success. The flower damage and subsequent drop rate
due to this beetle feeding is 23%.
Fruiting behavior
Pollinated
and fertilized flowers initiate fruit development immediately and take six
weeks to produce mature fruits (Image 2f–h). Fruit is a leguminous pod, oblong,
50–60 mm long, smooth, inflated with a leathery pericarp. It is 1-seeded,
ovoid, 3cm long, hard and gray-black with a shiny testa (Image 2i). The pod is water-buoyant and dispersed
by tidal currents. The fruit
pericarp becomes fibrous, disintegrates gradually releasing the seed which in
turn finally settles in the soil for germination and subsequent production of a
new plant.
DISCUSSION
InCaesalpinia genus, only two species, namely, C. bonduc and C.
crista extend their distribution into beach vegetation and are frequent
mangrove associates. C. bonduc is a pantropical, coarse scrambling vine
and has a wide distribution and such distribution is partly due to floating of
its seeds in tidal waters and to the extended viability of seeds in water. C. crista is also a
climber but is restricted to Asian beaches and mangroves (Tomlinson 1986). In Coringa mangroves, C. bonduc does
not occur while C. crista grows profusely landward. The landward occurrence is an indication
that this plant species is oligohaline with low salinity. The plant does not show up during the
summer season due to the shedding of aerial parts. The summer monsoon showers trigger the
leaf flushing process in this species, and once initiated, it continues into
the rainy season. This event is
immediately followed by flowering which extends into November. The leaf shedding occurs following the
maturation of fruits in late winter. These sequential events are characteristics of C. crista and
hence it is a typical seasonal plant. As a mangrove associate, C. crista is important for shoreline
stabilization to bind the soil together. Its prolific growth during the rainy
season stabilizes the soil and plays a prominent role in reducing soil erosion
and landward penetration of seawater.
The
genus Caesalpinia is pantropical and has a complex taxonomic history
with more than 120 terrestrial species most of which are known for their
ornamental and ecological values (Lewis 1998). The genus is poorly investigated
concerning its reproductive biology. A few species have been studied for understanding their pollination
biology and these studies are also mostly limited to the New World species
(Lewis 1998; Lewis & Gibbs 1999). The available information on pollination biology on Caesalpiniaspecies indicates the operation of different pollination syndromes. C. calycina is andromonoecious
while C. pluviosa is hermaphrodite; both are pollinated by carpenter
bees (Lewis & Gibbs 1999). C.
coluteifolia, C. exilifolia, C. paraguariensis and C. eriostachys
are all hermaphrodites and pollinated by carpenter bees (Jones & Buchman
1974). C. brevifolia, C. coriaria, C. echinata, C. sappan and C.
spinosa are bee-pollinated (Roubk 1995). C. pulcherrima is psychophilous
and pollinated by a diverse species of diurnal butterflies (Cruden &
Hermann-Parker 1979). Nocturnal
hawk moth pollination is reported in C. gilliesii (Cocucci et al. 1992),
hummingbird-pollination in C. exostemma and C. conzattii (Vogel
1990; Borges et al. 2009), and bat-pollination in C. bahamensis (Koch et
al. 2004). These reports indicate that Ceasalpinia species have
different flower types adapted to different pollinator types suggesting that
the floral characteristics in a given species are closely related to the
pollinator type (Vogel 1990). On
the contrary, based on floral characteristics and pollinator type, Lewis &
Gibbs (1999) reported that diverse groups occur within the genus Caesalpiniaand melittophily is the most common mode among studied species and hence, it is
likely to be the ancestral pollination syndrome. The changes in pollination
syndrome are likely to have been acquired independently by evolution from
melittophilous taxa within these groups after their differentiation from Caesalpinia.
Lewis et al. (2000) also stated that bee-pollination is largely widespread
among Caesalpinioideae and the Fabaceae in general (Arroyo 1981).
In
the present study, C. crista has a suite of floral characteristics
conforming to melittophily. These include diurnal anthesis, slight fragrance,
zygomorphy, yellow petals, with a flag petal displaying a conspicuous nectar
guide, and the presence of nectar with a high sugar concentration (Faegri &
van der Pijl 1979). C. echinataalso possesses the same characteristics as a melittophilous species (Borges et
al. 2009). High nectar sugar
concentration is reported in Pongamia pinnata (Raju & Rao
2006). In C. eriostachys,
the flowers are yellow and the flag petal with nectar guide absorbs ultraviolet
light (Jones & Buchmann 1974). The anthers absorb UV light while the filaments act as reflectors
(Borges et al. 2009). In C. crista, the nectar guide displayed by the
flag petal may be visible in ultraviolet light and Shi-Jin et al. (2004) also
felt the same. The petals, sepals and anthers are all yellow and they could
effectively be perceived by bees (Percival 1965). Further, extra-floral nectar along the
rachis is an additional attractant and is easily perceivable by bees. The pollinator attraction appears to be
a consequence of combination of floral and extra-floral nectar apart from the
primary floral characteristics (Shi-Jin et al. 2004). Restricted access to nectar through
openings between the bases of three upper stamens indicates resource
protection, a trait that Arroyo (1981) attributed to the more advanced genera
of Caesalpinioideae. This floral
specialization to restrict nectar is an important trait in melittophilous
species in which bees with their skills handle the flowers efficiently to
access the nectar in C. crista. Similar floral specialization to protect
nectar resource from unwanted foragers has also been reported in C. echinataand also in other species of Caesalpinia (Li et al. 2004).
In
the present study, C. crista with melittophilous characteristics is
pollinated almost exclusively by bees, especially carpenter bees. Further, a butterfly species, Catopsilia
pomona also visits the flowers for nectar suggesting that the plant does
not exclude visits by other types of visitors or occasional pollinators. Borges
et al. (2009) also reported that the melittophilous species, C. echinatadoes not exclude infrequent visits by other types of visitors or occasional
pollinators such as butterflies and hummingbirds. Further, wind also plays an important
role in the pollination of C. crista. The floral characteristics such as free
petals, fully exposed stamens with dry and powdery pollen grains and hairy
stigma favour anemophily. The
locations of this plant are windy during the rainy season and wind drives the
pollen effectively. The prolific
growth and near synchronous flowering at population level contribute to pollen
availability in huge quantities and enable anemophily as an effective mode of
pollination. The function of a
pollination system involving both bees and wind as vectors of pollen transfer
is referred to as ‘ambophily’ (Culley et al. 2002) and this pollination
system is adaptive for C. crista to set fruit in the absence of bees. Li
et al. (2004) and Shi-Jin et al. (2004) reported that C. cristain China is ambophilous and the biotic pollinators included honey bees,
carpenter bees, wasps, flies, ants, butterflies and coleopterans. Therefore, C. crista is
characteristically ambophilous and utilizes autochthonous pollinators,
especially bees for pollination. The bees are highly efficient in transferring
pollen and the same is realized in the body washings of bees which were
collected while foraging at the flowers.
InCaesalpinia genus, both self-incompatibility and self-compatibility has
been reported by different workers in the studied taxa. The self-incompatible
species include C. eriostachys (Bawa & Webb 1984; Bullock 1985), C.
coriaria, C. sclerocarpa (Bullock 1985), C. caladenia, C. pulcherrima(Bullock 1985) and C. echinata (Borges et al. 2009). The self-compatible
species include C. calycina (Lewis & Gibbs 1999), C. brevifolia,
C. echinata, C. sappan and C. spinosa (Roubik 1995). Further, Bawa
& Webb (1984) suggested that reports of self-incompatibility in
caesalpinioid species should be reassessed; the self-incompatibility in the
reported taxa is functional during post-zygotic stage only. In C. crista, the flowers are
bisexual, self-compatible and self-pollinating but it is principally
out-crossing and the same is realized in the hand-pollination tests. The difference in fruit set rate in
autogamy and geitonogamy could be related to minor genetic differences between
flowers of the same plant (Roubik 1995). The high pollen-ovule ratio evidenced in this plant also favours
out-crossing, sensu Cruden (1977). The ability to set fruit through self- and cross-pollination facilitatesC. crista for achieving genetic variation in order to survive in its
natural harsh environment where insects are not reliable throughout the
duration of flowering season. The
long period of flowering season is an additional advantage for more fruit
set. Despite the ambophilous
pollination syndrome, mixed breeding system and extended flowering season,
natural fruit set does not exceed 10%. The low fruit set evidenced in this plant is partly due to
flower-feeding by the beetle, Mylabris phalerata; its flower-feeding
rate stands at 23% at the study area. The extended flowering and nutrient resource constraints appear to be
regulating the fruit set rate in C. crista. Bawa & Webb (1984) and
Lewis & Gibbs (1999) reported low fruit set rates in C. pluviosa(4%), C. calycina (12.7%), C. gilliesii (12%) and C.
pulcherrima (13%). These
authors also mentioned that in C. eriostachys, fruit set is reduced due
to high rates of abortion of flowers and immature fruits. Stephenson (1981)
reported a low fruit set rate in C. echinata due to a high rate of
abortion as a consequence of pollination failure, availability and allocation
of nutrient reserves, sexual selection, or predation, among other factors. He
found a high incidence of seed and ovule abortion and seed predation even among
fruits that successfully developed as well as the presence of lepidopteran
larva inside many flowers. Leite
(2006) noted a high percentage of fruits without developed seeds in C.
pyramidalis. In C. crista,
bud, flower and fruit abortion is completely absent and the fruit set rate
evidenced in this plant is a consequence of flower-feeding by the beetle, high
investment in flower production over a period of three months at individual
level and the level of nutrients availability to the plant during fruiting
period.
InC. crista, the fruits mature within six weeks and each fruit produces a
single seed due to single-ovuled flowers. The fruits are indehiscent and disperse in that form in tidal
waters. They are water-buoyant but
they do not disperse far away from the parental sites since the species is
restricted to the landward side. The fruit pericarp being leathery in nature gradually disintegrates in
water releasing the seeds into water or soil. The seeds are also water-buoyant but
their dispersal occurs during the summer season when the soil is relatively
exposed and partly wet. They remain
dormant throughout the summer season during which they may be subjected to
desiccation and predation by soil insects; the healthy and viable seeds
germinate and produce new plants during the rainy season. Field studies indicated the emergence of
a few new plants each year suggesting that C. crista has recruitment
problems. Therefore, studies on seed predation and viability are suggested in
order to understand the seed and seedling ecology of this plant.
CONCLUSIONS
Caesalpinia crista as a mangrove associate is an important constituent in the
Coringa Mangrove Forest. It has its
share in soil binding and stabilization of the floor in the landward marginal
areas. The timing of the flowering
event and the intense flowering facilitates the local bees to use the flowers
as important sources of pollen and nectar. The hermaphroditic sexual system, self-compatibility, mixed breeding
system and anemophily is inevitable for the plant to fruit in the presence or
absence of pollen vectors since the latter are not reliable in the harsh
environment that exists in the mangrove ecosystem. The dual pollination system of ambophily
appears to have evolved over a period of time to cope with the adverse habitat
conditions in order to build up the population and expand the distribution in
the landward areas. The patchy
distribution or the occurrence of pure stands of C. crista in essence
sustains the local bees for almost half of the year and its habitat provides
the nesting sites for the soil burrowing bees. Therefore, the information provided in this
paper forms the basis for further work on seed ecology and seedling
establishment patterns within the mangrove forest ecosystem in relation to
salinity and nutrient levels in the soil.
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