Four Helvella(Ascomycota: Pezizales: Helvellaceae) species
from the Cold Desert of Leh, Ladakh,
Jammu and Kashmir, India
Konchok Dorjey1, Sanjeev Kumar 2 & Yash Pal Sharma 3
1,2,3 Department of Botany,
University of Jammu, Jammu, Jammu and Kashmir 180006, India
1 dorjeyusa@gmail.com, 2 sanjeevkoul222@gmail.com,3 yashdbm3@yahoo.co.in (corresponding author)
doi: http://dx.doi.org/10.11609/JoTT.o3057.3981-4
Editor: B.C. Suman,
University of Horticulture and Forestry, Solan, India Date of publication: 26 March 2013
(online & print)
Manuscript details: Ms #
o3057 | Received 06 January 2012 | Final received 07 August 2012 | Finally
accepted 17 February 2013
Citation: Dorjey,
K., S. Kumar & Y.P. Sharma (2013).Four Helvella (Ascomycota: Pezizales: Helvellaceae) species
from the Cold Desert of Leh, Ladakh,
Jammu and Kashmir, India. Journal of Threatened Taxa 5(5):
3981–3984; doi:10.11609/JoTT.o3057.3981-4.
Copyright: © Dorjeyet al. 2013. Creative Commons Attribution 3.0 Unported License. JoTTallows unrestricted use of this article in any medium, reproduction and
distribution by providing adequate credit to the authors and the source of
publication.
Funding: Junior Research Fellowship (Konchok Dorjey) from the
University Grants Commission (UGC), New Delhi.
Competing Interest: None.
Acknowledgements: authors
wish to thank Head, Department of Botany for providing laboratory facilities
and the first author acknowledges the financial assistance received as Junior
Research Fellowship from the University Grants Commission, New Delhi.
For
figures, images -- click here
The genus Helvella is a small assemblage of ascomycetous macrofungal species currently placed in the family Helvellaceae of order Pezizalesof sub-class Pezizomycetidae; class Pezizomycetes, sub-division Pezizomycotinaof division Ascomycota. Kirk et al.
(2008) included 52 species while Index Fungorum(2011) has entered 106 records in the genus so far. Across the world, species of Helvella have been reported from Europe and
North America (Anderson & Ickis 1921; Dissing
1964, 1966a,b; Dissing & Lange 1967; Kempton & Wells 1970). As many as 12 species of Helvella are known to exist in India (Lloyd
1904–1919; Sohi et al. 1965; Kar & Maity 1970; Bilgrami et al. 1991; Joshi et al. 1982; Jamaluddin et al. 2004), out of which seven species have
been reported from Jammu and Kashmir (Kaul 1971,
1981; Kaul et al. 1978; Abraham 1991; Kumar &
Sharma 2010). The present paper
deals with four Helvella species from the
district of Leh in Ladakhregion of the state of Jammu and Kashmir. Of these, three species, viz., Helvella acetabulam, H. corium and H. queletii are new records for India while H. macropus is the first authentic record
from Ladakh region.
Materials and Methods
Ladakh region in the state of Jammu
and Kashmir represents an important biogeographic province within Indian
Trans-Himalaya lying between 32015’–360N & 75015’–80015’E,
and located at an altitude of 3,649m (Fig. 1). The region exhibits unique topography,
intense climatic conditions of low annual precipitation (80–300 mm),
excessive dryness, extreme heat and cold (i.e., cold arid zone), intense solar
radiation with high wind velocity and very low relative humidity (40–45 %). During winter, the entire area is
covered with snow and temperature drops to as low as -250C. Specimen collections were made during
July and August 2010 from different locations of LehDistrict of Ladakh. Careful records were made of the habit,
habitat, and substrates of these macrofungi in the
field. Macroscopic and microscopic
features of ascocarps are based on fresh
specimens. Colournotations are as per Ridgway (1912). For microscopic characters, a small section of specimen was first kept
in 3% KOH to rehydrate the dried tissue and then stained in 1% Congo red and 1%phloxine. Microscopic characters were microphotographedusing a Sony N50 camera attached to a Olympus CH 20i
binocular microscope, and measurements were made for each character for
description of average dimension. For identification and description, works of Soothill& Fairhurst 1978; Smith et al. 1981; Arora 1986; Purkayastha &
Chandra 1985; Kumar et al. 1990; Atri et al.
2003 were employed. The examined
specimens have been deposited in the herbarium of Botany Department, University
of Jammu with accession numbers (BHJU).
Results and Discussion
1. Helvella acetabulum (L.) Quél.,
Hyménomycètes (Alençon):
102 (1874)
Synonymy: Acetabula sulcata (Pers.) Fuckel,
Jb. nassau. Ver. Naturk. 23–24: 330 (1870) (1869–70)
Macroscyphus acetabuliforme Gray, Nat. Arr. Brit. Pl.(London) 1: 772 (1821)
Paxina acetabulum (L.) Kuntze,Revis. Gen. pl. (Leipzig) 2: 864 (1891)
Peziza acetabulum L., Sp. pl. 2: 1181
(1753)
Peziza sulcata Pers., Syn. meth. fung. (Gottingen) 2: 643
(1801)
Material examined: BHJU 182, 05.vii.2010, Humicolous,
scattered, mixed forests of Salix excelsa, S.
alba, Populus nigra and P. caspica, Wanlaarea, Leh, Ladakh, Jammu
and Kashmir, coll. K. Dorjey, S. Kumar and Y.P.
Sharma (Image 1 a,b &
Image 2 a–c).
Carpophore: 3.0–3.8 cm
in diameter, saucer or cup shaped, grey-brown (vintage walnut), buffy olive
towards outer, margins slightly splitted, smooth,
ribs prominent, whitish to creamish, extending nearly
to the margin of the cup; stipe: 3.0–3.2 cm in length, short, white,
broader to the apex and narrow towards end, interior chambered; ascospore: 12.8–16.8 × 11.2–13.6 µm, sub-globose to oval, thin walled, shiny white, smooth, mono to multigattullate; asci:
160.0–232.0 µm long, 12.0–14.0 µm wide at the top, 14.0–16.0
µm at the middle and 6.0–10.5 µm at the base, elongated cylindrical,
hyaline, thin walled, each ascus contain eight ascospores; paraphysis:
3.2–4 µm, elongated, thin-walled, hyaline, septate,
bulbous at the tip; pubescent hairs: 6.0–18.0 µm, septate,
branched.
Edibility: Not known
2. Helvellacorium (O. Weberb.) Massee, Brit.-Fl 4:463 (1895)
Synonymy: Cowlesia corium (O. Weberb.)Nieuwl., Am. Midl. Nat. 4: 380 (1916)
Cyathipodia corium (O. Weberb.)Boud., Hist. Class. Discom. Eur. (Paris): 39 (1907)
Lachnea corium (O. Weberb.)
W. Phillips, Man. Brit. Discomyc. (London):
204 (1887)
Paxina corium (O. Weberb.)
W. Seaver, North American Cup-fungi,
(Operculates) (New York): (1928)
Material examined: BHJU 185, 03.viii.2010, Humicolous,
scattered, mixed forest of Salix alba, S. excelsa. Populus nigra, Wanla area, Leh, Ladakh, Jammu and Kashmir,
coll. K. Dorjey, S. Kumar and Y.P. Sharma (Image 1 f,g & Image 2 i–l).
Carpophore: 1–2.5 cm in
diameter, blackish-brown, from inside as well as outside, depressed, surface
smooth, dull, dry, crenate; stipe: 1.5–2.0 cm in length, solid, concolorous with carpophore; ascospore:
14.4–20.0 × 8.8–11.2 µm, rounded ellipsoid, thin walled,
smooth, hyaline with a large central oil droplet; asci:
204–280 µm long, 12.0–20.0 µm wide at the top, 14.0–18.0 µm
at the middle and 8.0–14.0 µm at the base, elongated, cylindrical, thin
walled, hyaline, each ascus contain eight ascospores, arranged uniseriateto obliquely placed; paraphysis: 4.0–6.0 µm
wide, slender with bulbous at the tip, septate,
thin-walled, hyaline; pubescent hair: 12.0–18.0 µm wide, brown, septate, branched.
Edibility: Not known
3. Helvella macropus (Pers.) P. Bidr. Känn. Finl. Nat. Folk 19:
37 (1871)
Synonymy: Cyathipodia macropus (Pers.) Dennis, British
Cup Fungi & their Allies: 7 (1960)
Helvella bulbosa (Hedw.)Kreisel, Boletus, SchrReihe1: 29 (1984)
Lachnea macropus (Pers.) W. Phillips, Man.
Brit. Discomyc. (London): 207 (1887)
Macropodia bulbosa (Hedw.)
Fr., Syst. Mycol. (Lundae) 2(1): 58 (1822)
Macroscyphus macropus (Pers.) Gray, Nat. Arr.
Brit. Pl. (London) 1: 372 (1821)
Octospora bulbosa Hedw., Descr. micr.-anal. musc. frond. 2: 34
(1789)
Material examined: BHJU 186, 05.vii.2010, humicolous, scattered,
mixed forests of Salix excelsa, S. alba, Populus nigraand Mentha longifolia,Wanla area, Leh, Ladakh, Jammu and Kashmir, coll. K. Dorjey,
S. Kumar and Y.P. Sharma (Image 1c & Image 2d–f).
Carpophore: 1.2-2.8 cm in
diameter, cup-shaped, hymenium dark grayish-brown ribs prominent, whitish to
cream colour, extending nearly to the margin of the
cup; stipe: 1.5–2.5 cm long, slightly swollen near the base, upper part concolorous, below paler, smooth; ascospore:
14.7–17.6x8.0–10.4 µm, sub-globose to
ellipsoidal, thin-walled, shiny white; asci:
200.0–280.0 µm long, 10.0-12.0 µm wide at the top, and 12.5-16.0 µm at
the middle and 6.5–9.5 µm at the base, elongated cylindrical, hyaline,
thin walled, each ascus contain eight ascospores; paraphysis:
4.0–6.0 µm wide, elongated, thin walled, hyaline, septate;
pubescent hairs: 6.0–26.0 µm, septate,
branched.
Edibility: Not known.
4. Helvella queletii Bres., Fung. trident. 1(3): 39
(1882)
Synonymy: Acetabulaqueletii (Bres.) Benedix, Kulturpflanze 10:
365 (1962)
Material examined: BHJU 190, 07.vii.2010, humicolous,
scattered, mixed forests of Populus nigra, Prunus armeniaca, Wanla area, Leh, Ladakh, Jammu and Kashmir,
K. Dorjey, S. Kumar and Y.P. Sharma (Image 1 d,e & Image 2 g,h).
Carpophore: 4.5–5.0 cm
in diameter, saucer or cup shaped, hymenium smoke gray, margins entire, smooth,
exterior creamish; stipe: 2.0–2.5 cm long,
short, white with branching ribs that don not extend onto the cup, white; ascospore: 10.0–16.0×8.0–12.0 µm, sub-globose to oval, thin walled, shiny white; asci: 200–240 µm long, 12.0–14.0 µm wide at the
top, 12.5–18.0 µm at the middle and 6.0–10.0 µm at the base,
elongated cylindrical, hyaline, thin walled, each ascuscontain eight ascospores; paraphysis:
4.0–6.0 µm wide elongated, thin walled, hyaline, septate;
pubescent hairs: 8.0–16.0 µm wide, septate,
branched.
Edibility: Not known.
The survey of literature (Purkayastha & Chandra 1976, 1985; Bilgramiet al. 1991; Sarbhoy et al. 1975, 1996; Jamaluddin et al. 2004) reveals that Helvella acetabulum, H. corium and H. queletii are new additions to Indian macrofungi while H. macropus constitutes first authentic record from Ladakh region of the state of Jammu and Kashmir.
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